Overview |
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Product |
Camelid PBMC isolation KitTM |
Description | Camelid Peripheral blood mononuclear cells (PBMCs) isolation kit is a ready-to-use kit containing sterile dilution buffers, density mediums and density cushions for the isolation of PBMC cells from whole blood of various camel family members including Llama, Alpaca. |
Contents |
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PBMC SepOBufferTM | 150 mL |
PBMC Dilution Buffer | 250 mL |
RBC Lysis Buffer | 50 mL |
PBMC separation tubes with medium grit frit | 10 tubes |
Differences in cell density are exploited to separate granulocytes and erythrocytes from peripheral
blood mononuclear cells (PBMCs). The most common technique for separating PBMCs is to mix blood
with a compound which aggregates the erythrocytes, thereby increasing their sedimentation rate. The
active ingredients in the SepOBuffer™ are sodium diatrizoate and polysaccharide with osmolality
290 +15 mOsm/L and density of 1.077 +0.001 g/ml. The polysaccharide in SepOBuffer™ enhances
erythrocyte aggregation increasing erythrocyte sedimentation during centrifugation. PBMCs with lower
densities remain in the plasma SepOBuffer™ interface and can be collected from the upper part of the
tube when the erythrocytes have settled. The PBMCs from whole blood can be reliably and rapidly
isolated in pure form using SepOBuffer™.