Overview |
|
Product |
SolOBufferTM CellLysisKit |
Description | SolOBufferTMCellLysisKit utilizesa speciallyformulatedcelllysisbufferthatusesnon-denaturingdetergentstopreparecelllysatesforBIOLOGicalassays. |
Volume | 200mLSolObufferTM;100lProteaseinhibitorcocktailAandB(each). |
Storage | Storeat4⁰C. StoreProteaseinhibitorcocktailAandBat-20⁰C. Approximateshelflifeissixmonths |
Applications | ProteincharacterizationandEnzymaticactivityassays. |
Advantages |
|
- Perfectforvarietyoftechniquesincludingproteincharacterizationandenzymatic
activitydetermination - Readytouseaftercentrifugation-Nodetergentremovalrequired
- Preserveproteinsamples
- Non-Denaturing
- Eliminateissueswithtroubleshootingassays
- Kitincludesproteaseinhibitorstopreservebiologicalsamples
Protocol |
|
- Recommendedvolumefortissueproteinextractionis4-6mL/goftissueor
1-2mLfor106cells. - AliquotdesiredvolumeofSolOBufferTM.
- Add1µLofProteaseinhibitorAand1µlofProteaseinhibitorBto2mLofSolOBufferTM
immediatelybeforeuse. - ProteaseinhibitorsarenotstableinSolOBufferTM,sobesuretoonlymakewhatisneeded.
- AdddesiredvolumeofSolOBufferTMtothesampleanddisruptthetissues/cellsvia
mechanicaldisruptionorsonicationfor5seconds. - Keepsamplesonicefor15minutes.
- Centrifugefor3minutesat12000XG(microfuge)
- Insomecaseswheresmallpelletmayremainwhichcontainscelldebrisand
otherinsolublematerial. - SolubilizedsamplecanbeuseddirectlyinWesternBlotandImmunoprecipitation
withoutneedtoremoveanydetergents.