Overview |
|
Product |
StripOBufferTM |
Description | StripOBufferTMisaspeciallyformulatedwaterbasedbufferthatwillstripantibodiesfromantigensin5-10minutesatroomtemperature |
Volume | 100ML |
Concentration | 10Xsolution |
Storage | Roomtemperatureforshort-termstorage |
Applications | 25mLofStripObuffercanbeusedon25-50mini-blots. |
Advantages |
|
- Savetimebyre-usingblots
- Preserveproteinandantigensamples
- EliminateissueswithproperequalproteinloADIng
- Comparephosphorylatedandnon-phosphorylatedstateofthesameprotein
onthesameblot - Reconfirmproteinexpressionbyre-probingthesameblotwithdifferentsetof
antibodiesraisedagainstantibodiesfromdifferentvendorsorsameordifferentepitopes - Preformmultiplestripping’swhilemaintainingantigenicity
- Noheatingrequired
- Noβ-mercaptoethanolrequired–Nosmell
- CompatIBLewithNitrocelluloseandPVDFmembranes
Protocol |
|
- Dilute(1:10)thesuppliedbufferindistilledwaterpriortouse.
- Rinsethemembraneindistilledwaterfirstandtheninstrippingbufferatleastonce.
Multiplerinseswithbufferresultsinclearerbandsonautoradiograms - Immersethemembranecompletelyin10mLofstrippingbufferforeach
mini-gelmembrane. - Gentlyshakethemembraneonarockerfor5-10minutesatroomtemperature.
- Rinsethemembranewithdistilledwater.
- RinsethemembranewithTBSTorthebufferinwhichyourprimaryantibodyisdiluted.
- Proceedforblockingstage.