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蚂蚁淘在线 / 品牌中心 / FabGennix / FabGennix/PDEase Kit/PDEase-100

FabGennix/PDEase Kit/PDEase-100

价格
￥7900.00
货号：PDEase-100
浏览量：127
品牌：FabGennix
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Overview

Product

PDEase KitTM

Description

PDEase KitTM is designed to allow investigators to determine PDE4 activity

Contents

Incubation Buffer Reagent A

5 mL

Incubation Buffer Reagent B

5 mL

Incubation Buffer Reagent C

5 mL

PDE Substrate Buffer D

10 mL

PDE Substrate

2X 200 µl

Inhibitor Buffer

50 mL

Second Enzyme Source (10X)

2X 250 µl

Resin Slurry (10X)

100 mL

Control PDE4 Enzyme

1x200 µl

Introduction

cAMP is a second messenger that is ubiquitously found in all mammalian cells. This messenger modulates the signal transduction of a variety of hormones, neurotransmitters, growth factors, cytokines and others ligand receptors. cAMP phosphodiesterase enzymes provide the sole pathway for hydrolyzing and truncating the cAMP signaling that is originated in response to receptor activation. The cAMP hydrolyzing activity is provided by a fairly large multi-enzyme family with distinct intracellular localization and regulatory properties. Among various PDE families (PDE1-PDE11), PDE4 has 4 genes (PDE4A, PDE4B, PDE4C, PDE4D) that are expressed in multi-splice variant forms.

The PDE4 activity measurement required a 2 step reaction, in the first reaction, cAMP is hydrolyzed by PDE activity in to non-cyclic form. This reaction is stopped by denaturing the PDE source or by addition of PDE4-selective inhibitor, followed by conversion of AMP to adenosine by the second enzymatic reaction to equilibrium. Since Adenosine is neutral, cAMP or AMP is separated by charge by ion exchange chromatography. In order to avoid background contribution of other PDEs, this PDE assay is carried out at substrate concentration optimized for PDE4. The PDE4 enzymatic activity is directly proportional to the adenosine formed in the first reaction.

This PDE4 activity measurement provides reproducible results within 2-3 hours. The kit provides enough reagents to process 50 or 100 samples in tandem along with internal controls. The kit reagents are stable for 6 months when stored at recommended conditions. The contents of this kit must be stored at different temperatures as indicated on the bottle labels and specification sheet.

PDEase Kit Protocol

Fabgennix 品牌介绍 官网：http://www.fabgennix.com/   Fabgennix公司是一家知名的抗体研发生产企业，为全球生物技术公司、科研单位和生物制药公司提供一抗、二抗、试剂、试剂盒、多肽和阳性质控品等产品。 基于坚实的科研背景，Fabgennix公司已成为全球免疫诊断市场高质量单克隆抗体的优质供应商。       Fabgennix 主要产品分类     【一抗】 1. 癌症研究 2. 免疫学 3. 微生物学 4. 信号转导 5. 新陈代谢和内稳态 6. 视力和嗅觉 7. 通道和转运蛋白 8. 表观遗传学 9. 干细胞 10. 细胞生物学 11. 神经病学 12. 心脏病学 13. 内分泌系统 【二抗】 1. Rabbit Anti-Chicken IgG HRP 【试剂】 1. Western Blot Stripping Buffer 2. 硝酸纤维膜蛋白染料 【试剂盒】 1. 细胞溶解试剂盒 2. PDEase 试剂盒 3. 外周血单核细胞（PBMC）分离试剂盒 【实验室服务】 1. 客户抗体显影 2. 蛋白化学多肽合成实验室 3. 免疫学实验室 4. 影像处理实验室 5. 免疫组化实验室