General Information

Product

VPS29 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 133-183 on rat Vacuolar Protein Sorting-Associated Protein 29.

Accession #

NCBI: NP_001099402.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-1.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9UBQ0

Overview

Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3.

Molecular Function

Metal ion binding, Phosphoserine phosphatase activity

Subcellular Location

Cytoplasm. Membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Early endosome Curated. Late endosome.

Expression

Ubiquitous. Highly expressed in heart, lung, placenta, spleen, peripheral blood leukocytes, thymus, colon skeletal muscle, kidney and brain.

Structure

Component of the heterotrimeric retromer cargo-selective complex (CSC), also described as vacuolar protein sorting subcomplex (VPS), formed by VPS26 (VPS26A or VPS26B), VPS29 and VPS35 (PubMed:11102511, PubMed:16737443, PubMed:17891154). The CSC has a highly elongated structure with VPS26 and VPS29 binding independently at opposite distal ends of VPS35 as central platform. The CSC is believed to associate with variable sorting nexins to form functionally distinct retromer complex variants.

Alternative Nomenclature

DC15 antibody

DC7 antibody

DKFZp564F0223 antibody

FLJ20492 antibody

hVPS29 antibody

PEP11 antibody

Retromer protein antibody

Vacuolar protein sorting 29 antibody

Vacuolar sorting protein VPS29/PEP11 antibody

Vesicle protein sorting 29 antibody

VPS 29 antibody

x 007 protein antibody