General Information

Product

ergo-1 Antibody

Description

Affinity Purified Endogenous-RNAi deficient arGOnaute antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Caenorhabditis elegans

Immunogen

Synthetic peptide taken within amino acid region 790-840 on C.elegans Endogenous-RNAi deficient arGOnaute protein.

Accession #

NCBI: NP_503362.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.62-0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

O61931

Overview

Argonaute protein required for gene silencing in the endogenous RNA interference (RNAi) pathway. Interacts with 26G small interfering RNAs (26G-siRNAs) and required for subsequent biogenesis of secondary 22G-siRNAs. May be involved in passenger strand cleavage of target 26G-siRNAs(PubMed: 22102828).

Molecular Function

Dead/H-box RNA helicase binding, nucleic acid binding, production of siRNA involved in RNA interference, regulation of translation, RNA interference.

Subcellular Location

Cytoplasm

Structure

Belongs to the argonaute family. Piwi subfamily. Contains 1 PAZ domain. Contains 1 Piwi domain.

Alternative Nomenclature

Endogenous RNAi deficient argonaute antibody

Ergo 1 antibody

Hypothetical protein R09A1.1 antibody