Description F2H^®-Kit Basic allows studying interactions between GFP- and RFP-tagged proteins in live mammalian cells.

Specificity Bait: GFP-fusion protein Prey: RFP-fusion protein, mCherry-fusion protein Tested on cytoplasmic, nuclear, and mitochondrial proteins Cell line: BHK (baby hamster kidney)

Application Visualize protein-protein interactions inside cells Validate your pull-down results or Y2H hits in mammalian cells Identify interacting protein domains

Principle

• F2H cells (BHK origin) carry a GFP-anchoring platform in the nucleus
• GFP- and RFP-fusion proteins (bait and prey) are transiently expressed in F2H cells
• GFP-bait is recruited to the platform → bright green spot
• If RFP-prey interacts with the bait → red fluorescent spot co-localizes with the green spot
• If RFP-prey does not interact with the bait → no red spot, disperse red fluorescence

ChromoTek常规抗体系列   Dnmt1 antibody [2E8]   GFP antibody [3H9]   GST antibody [6G9]   HA antibody [7C9]   Myc-tag antibody [9E1]   PCNA antibody [16D10]   RFP antibody [5F8]   RFP antibody [6G6]   RNA Pol II