Description Analyze and manipulate the intramolecular interaction between the N- and C-terminal domains of Androgen Receptor (AR) with ChromoTek Fluorescent-Two Hybrid (F2H^®) AR. Wild-type (wt), as well as several mutant AR forms can be investigated.

Specificity AR is expressed as two separate domains Bait: AR ligand binding domain (AR-LBD) fused to GFP Prey: AR N-terminal domain (AR-NTD) fused to RFP Bait and prey interact.

Applications Detect early steps of Androgen Receptor activation Real-time agonists and antagonists screening Visualized in live mammalian cells

Available AR variants AR-LBD: wt, W741L, F876L, T877A, F876L-T877A

Click on the left picture for a live cell analysis example Live-cell F2H imaging of reversible interactions between AR-LBD-GFP (green) and AR-NTD-RFP (red) in F2H-BHK cells

ChromoTek常规抗体系列   Dnmt1 antibody [2E8]   GFP antibody [3H9]   GST antibody [6G9]   HA antibody [7C9]   Myc-tag antibody [9E1]   PCNA antibody [16D10]   RFP antibody [5F8]   RFP antibody [6G6]   RNA Pol II