Comparison of BlastR™ versus commonly used non-denaturing and denaturing lysis buffers. Isolation of proteins from the membrane, cytoplasm, mitochondria, and nucleus.

A431 cells were lysed with BlastR™, RIPA, mPER, IP lysis, Denaturing (1% SDS), and Laemmli lysis buffers. Isolation of proteins from the membrane, cytoplasmic, mitochondrial, and nuclear markers were determined using the respective compartment marker proteins. The western blot figure highlights the ability of BlastR™ buffer to isolate proteins from all cellular compartments similarly to other denaturing buffers. Unlike ofther denaturing buffers, the BlastR™ buffer is compatible with standard protein assays, which is critical when equivalent protein loading is essential.  BlastR™ buffer is also an excellent IP buffer when used with the BlastR™ dilution buffer as outlined in the datasheet. These features are outlined in the table below for easy comparison with available lysis buffers.