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蚂蚁淘在线 / 品牌中心 / Cytoskeleton / Cytoskeleton/6xHis MsrB2 Protein aa: 42-182 (2 x 50 micrograms)/1 x 1 mg/MB201-XL

Cytoskeleton/6xHis MsrB2 Protein aa: 42-182 (2 x 50 micrograms)/1 x 1 mg/MB201-XL

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￥25000.00

货号：MB201-XL

浏览量：110

品牌：Cytoskeleton

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TheMsrfamilyarewellconservedproteinsthatfunctiontoreduceoxidizedmethioninetoL-methionine.TheSelR(MsrB)proteinwasidentifiedastheenzymeresponsIBLeforreductionoftheMICAL-oxidizedMet44andMet47aminoacidsofactin..AspartoftheMOXtrue™productline,humanMSRB2protein(MB201)[accession#Q9Y3D2(MSRB2_HUMAN)]hasbeenproducedandpurifiedfromabacterialexpressionsystem.TherecombinantproteinisN-terminally6xHistidinetaggedandiscomprisedofaminoacids42-182.ValidationstudieshaveshownthatMB201canreduceMICAL-oxidizedactinwhichwillenhancesubtilisinAcleavageby>90%inlimitedproteolysisassays.MB201hasalsobeenvalidatedfortheproductionofreducedactinwhichcanbeusedindownstreamapplicationssuchassedimentationassaysandactinpolymerizationassays.

TolearnmoreaboutusingMsrB2asaresearchtoolseeourdatasheet

TolearnmoreabouttheMICAL/MsrB/ActinphysiologicalredoxsystemseeourNewsletter

Eachlotofpurifiedproteinisqualitycontrolledtoprovidehighbatchtobatchconsistency,seeCOAdocuments.

ValidatedApplications

Formoreinformationcontact:signalseeker@Cytoskeleton.com

AssociatedProducts:

MOXtrue™6xHisMICAL-1Protein(Cat.#MIC01)

MOXtrue™MICAL-oxidizedActin(Cat.#MXA95)

MOXtrue™MICAL-oxidizedPyreneLabeledActin(Cat.#MXAP95)

RabbitSkeletalMuscleActin(Cat.#AKL95)

PyreneLabeledRabbitSkeletalMuscleActin(Cat.#AP05)

Cytoskeleton运动蛋白Cytoskeleton Motor Werks™（CMW）产品线由Cytoskeleton独家制造和销售。这些产品促进了运动蛋白领域的研究和药物开发（Funk et al。2005）。我们致力于生产真核和真菌来源的高纯度和具有生物活性的驱动蛋白和肌球蛋白家族蛋白。这些试剂旨在用于抗有丝分裂药物的发现和运动活性的机理研究。Cytoskeleton Motor Werks™系列产品还包含几种Biochem Kits™，抗体和其他与运动相关的试剂（例如，微管稳定剂紫杉醇，目录号TXD01）和蛋白质（例如，预先形成的微管，目录号MT002）。有关运动蛋白的更多信息，请单击上方的关于标签。从以下类别中选择：药物和缓冲液F-肌动蛋白丝套件微管蛋白质类

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MsrB2ProteinPurityDetermination A10μgsampleofMsrB2proteinwasseparatedbyelectrophoresisina4-20%tris-glycinegelandstainedwithCoomassieBlue.ProteinquantitationwasperformedusingthePrecisionRed^TMProteinAssayReagent(Cat.#ADV02).Mark12standardmolecularweightMarkersarefromInvitrogen.

SubtilisinAssayonMsrB2TreatedOxidizedActin MICAL-oxidizedactin(MetOActin)(Cat#MXA95)wasdilutedto0.1mg/ml(2.3mM)andleftuntreated,ortreatedwithDTT(10mM),MB201(0.3mM),oracombinationofboth(seemethod).2mgofeachsamplewasthenleftuntreated,ortreatedwithsubtilisin(1:200w/w)for15min.SampleswerethenseparatedbySDS-PAGEandvisualizedwithCoomassiestaining. Clickhereforadetailedmethod

OxidizedActinSedimentation+/-MsrB2Treatment MICAL-oxidizedactin(MetOActin)(Cat.#MXA95)wasdilutedto0.2mg/ml(4.6mM)or0.8mg/ml(18.4mM)(seemethod).Sampleswerethenincubatedwith2xpolymerizationbufferor2xpolymerizationbuffersupplementedwithMB201+DTTfor1hatroomtemperature.Sampleswerespuninanultracentrifugeat100,000gfor1.5h.SampleswerethenseparatedbySDS-PAGEandvisualizedwithCoomassiestaining. Clickhereforadetailedmethod

OxidizedActinPolymerization+/-MsrB2Treatment Pyrene-labeledactin(Cat#AP05)andMICAL-oxidizedpyrenelabeledactin(Cat.#MXAP95)weredilutedto0.1mg/ml(2.3mM)(seemethod).Sampleswerethenincubatedwith2xpolymerizationbuffersupplementedwithnothing(labeledControl),DTTalone(labeled+DTT),MB2010.3µM+DTT(labeled+MB2010.3),orMB2011.2µM+DTT(labeled+MB2011.2),.Uponactinpolymerizationfluorescencewasdetectedwithaspectrophotometer(seemethodbelow).A.U.=arbitraryunits Clickhereforadetailedmethod