Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process. Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE. Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin. Abbiotec提供了293个细胞的10,000多种裂解物，这些细胞被编码人类基因组25,000种蛋白质之一的质粒转染。裂解液是由用TrueORF基因携带的pCMV质粒（OriGene Technologies，Inc.）瞬时转染的HEK293T细胞制备的，然后在RIPA缓冲液中裂解。人全长蛋白带有C端Myc / DDK标签以进行检测。TACL™产品系列在Western印迹和免疫沉淀，ELISA标准品和反向蛋白质阵列中用作阳性对照。每种产品均包含纯载体转染裂解物作为阴性对照。