The kinesin superfamily proteins (KIFs) are microtubule-dependent motors that slide along microtubules and transport cellular organelles and mRNA to different parts of the cell. In neurons, KIF5 performs this role in an anterograde fashion from the neuronal cell body to axonal or dendritic terminals. KIF5 was initially differentiated from the ubiquitously expressed kinesin as being highly enriched in subsets of neurons and selectively concentrated in the cell body. KIF5 has at least three known isoforms; this antibody recognizes all three. Mutations in one of the isoforms of KIF5 (KIF5A) are thought to be involved in hereditary spastic paraplegias. Abbiotec提供了293个细胞的10,000多种裂解物，这些细胞被编码人类基因组25,000种蛋白质之一的质粒转染。裂解液是由用TrueORF基因携带的pCMV质粒（OriGene Technologies，Inc.）瞬时转染的HEK293T细胞制备的，然后在RIPA缓冲液中裂解。人全长蛋白带有C端Myc / DDK标签以进行检测。TACL™产品系列在Western印迹和免疫沉淀，ELISA标准品和反向蛋白质阵列中用作阳性对照。每种产品均包含纯载体转染裂解物作为阴性对照。