eNOS(EndothelialNOS)isanitricoxydesynthaseenzyme,partoftheNitricoxydepathway.Nitricoxidesynthases(NOS)areenzymesthatcatalyzethegenerationofnitricoxide(NO)fromL-arginineandrequirenicotinamideadeninedinucleotidephosphate(NADPH)asacofactor.AtleastthreeisioformsofNOShavebeenidenified:neuronalNOS(nNOS),endothelialNOS(eNOS),andinducIBLeNOS(iNOS).NOS-3(eNOS,endothelialNOS)producesNOwhichisimplicatedinvascularsmoothmusclerelaxationthroughacGMP-mediatedsignaltransductionpathway.NOmediatesvascularendothelialgrowthfactor(VEGF)-inducedangiogenesisincoronaryvesselsandpromotesbloodclottingthroughtheactivationofplatelets. Abbiotec提供了293个细胞的10,000多种裂解物，这些细胞被编码人类基因组25,000种蛋白质之一的质粒转染。裂解液是由用TrueORF基因携带的pCMV质粒（OriGene Technologies，Inc.）瞬时转染的HEK293T细胞制备的，然后在RIPA缓冲液中裂解。人全长蛋白带有C端Myc / DDK标签以进行检测。TACL™产品系列在Western印迹和免疫沉淀，ELISA标准品和反向蛋白质阵列中用作阳性对照。每种产品均包含纯载体转染裂解物作为阴性对照。