Product Information

Kit supplied with the following:

One (1) vial containing 50μL of fully biotinylated MBP-AviTag^TM fusion protein at 1mg/mL (50μg).

One (1) vial containing 100μL of unbiotinylated MBP-AviTag^TM fusion protein at 1mg/mL (100μg).

By comparing a known amount of a 100% biotinylated AviTag^TM fusion protein as a standard against a known amount of BirA-reacted protein-of-interest in an ELISA-type, multi-well dilution format (called BRTA for Biotinylation Reaction Titration Assay) it is possible to assess the extent of biotinylation achieved in your own BirA biotinylation reactions in a highly accurate and reproducible manner.

In addition, a mass shift between biotinylated and unbiotinylated fusion proteins visible on both SDS-PAGE and Western blot can be used to subjectively determine the amount of biotinylation that has occurred in a reaction by the observation of differences in band density in a mixed population of biotinylated vs. nonbiotinylated substrate.

The BIS-300 Kit contains the fully biotinylated standard used for the BRTA protocol and an unbiotinylated AviTag"d protein to react in a parallel to your BirA/protein biotinylation reaction as a positive control. A link to the BRTA protocol can be found here.

Avidity研发和销售用于分子连接的分子亲和工具，其的AviTag™技术采用针对性很强的一个独特的15氨基酸肽，用来自大肠杆菌的生物素连接酶（BirA）标签上的单一的生物素酶的共轭。   Avidity develops and sells molecular affinity tools for connecting molecules. Our patented AviTag™ technology employs a highly targeted enzymatic conjugation of a single biotin on a unique 15 amino acid peptide tag using the biotin ligase （BirA） from E. coli. AviTag™ sequence is GLNDIFEAQKIEWHE.  Oriented on streptavidin-coated surfaces, this creates an ideal presentation for molecular binding interactions. 公司：www.avidity.com   产品编号 产品名称 规格 价格（元） 生产 AbC Anti-C-terminus AviTagTM Antibody 100 ul ￥3,600.00 Avidity AbCA Anti-C-terminus AviTagTM –conjugated Agarose Resin 2 ml ￥4,500.00 Avidity AVB100 Efficient Biotinylation E. coli K12 Strain AVB100 1 vial ￥4,500.00 Avidity AVB101 Efficient Biotinylation E. coli B Strain AVB101 1 vial ￥4,500.00 Avidity AVB99 Efficient Biotinylation E.coli K12 Strain AVB99 1 vial ￥4,500.00 Avidity BIO200 Biotin Solution 1.5 ml ￥810.00 Avidity BIO500 Biotin Solution 10 ml ￥1,620.00 Avidity BIP300 Positive Control Plasmid 2 ug ￥2,700.00 Avidity BIRA500 BirA Biotin-Protein Ligase 40 ug ￥4,500.00 Avidity BIS300 Positive Control Protein Substrate Kit 1 kit ￥2,700.00 Avidity Bulk BIRA Bulk BirA Biotin-Protein Ligase 300 ug ￥17,640.00 Avidity CFT100 Mammalian Cell-Free Translation System: AvidExpressTM 1 kit ￥3,150.00 Avidity CTZ-1 Native Coelenterazine Free Base 1 mg ￥3,600.00 Avidity CVB101 Chemically Competent E.coli B Strain AVB101 10 vials ￥3,150.00 Avidity EVB100 Electrocompetent E. coli K12 Strain AVB100 10 vials ￥3,150.00 Avidity EVB101 Electrocompetent E. coli B Strain AVB101 10 vials ￥3,150.00 Avidity FASP Sequence Primer-Forward 200 pmol ￥1,710.00 Avidity Gluc-10 Biotinylated Gaussia princeps Luciferase 10 ug ￥1,350.00 Avidity Gluc-100 Biotinylated Gaussia princeps Luciferase 100 ug ￥6,300.00 Avidity pAC4 Biotin AviTagTM Vectors（C-terminal） 10 ug ￥2,970.00 Avidity pAC5 Biotin AviTagTM Vectors（C-terminal） 10 ug ￥2,970.00 Avidity pAC6 Biotin AviTagTM Vectors（C-terminal） 10 ug ￥2,970.00 Avidity pACS Biotin AviTagTM Vectors（C-terminal） 1 kit ￥8,100.00 Avidity pAN4 Biotin AviTagTM Vectors（N-terminal） 10 ug ￥2,970.00 Avidity pAN5 Biotin AviTagTM Vectors（N-terminal） 10 ug ￥2,970.00 Avidity pAN6 Biotin AviTagTM Vectors（N-terminal） 10 ug ￥2,970.00 Avidity pANS Biotin AviTagTM Vectors（N-terminal） 1 kit ￥8,100.00 Avidity RASP Sequence Primer-Reverse 200 pmol ￥1,710.00 Avidity rrGFP-100 Biotinylated Renilla reniformis Green Fluorescent Protein 100 ug ￥5,400.00 Avidity SA-Gluc Streptavidin-conjugated Gaussia princeps Luciferase 50 ug ￥4,500.00 Avidity SA-rrGFP 1.0 Streptavidin-conjugated Renilla reniformis Green Fluorescent Protein 160 ug ￥3,600.00 Avidity