Product Information

Kit supplied with the following:

• Two (2) vials of 20uL each 1mg/mL BirA biotin-protein ligase (40ug total)
• Two (2) vials of 1.5mL each BiomixA (0.5M bicine buffer solution, pH 8.3)
• Two (2) vials of 1.5mL each BiomixB (100mM ATP, 100mM Mg(OAc)2, 500uM d-biotin)
• Two (2) vials of 1.5mL each additional BIO-200 (500uM d-biotin)

The BirA biotin-protein ligase (EC 6.3.4.15) adds d-biotin covalently to biotin-acceptor peptides/proteins via an ATP intermediate (biotinyl 5"-adenylate) in a highly efficient and targeted manner. The downstream applications of enzymatically biotinylated proteins are varied, important and powerful. The well known biotin-avidin/streptavidin interaction is often exploited for affinity chromatography or protein immobilization on surfaces or substrates. Protein detection via anti-biotin antibodies or avidin/streptavidin-reporter enzyme conjugates (-HRP, -alkaline phosphatase) or fluorescent probes becomes possible. Multimeric forms of biotinylated MHC molecules are popular tools in immunobiology.

When used in combination with our AviTag™ biotin-acceptor peptide amino acid sequence, biotinylation occurs at twice the rate of the natural E. coli BCCP substrate and as much as an order of magnitude or more over other Biotinylation of Peptide sequences. The AviTag™ sequence consequently requires less of the BirA enzyme and shorter incubation times to biotinylate to completion than do other sequences available. If protein instability or protease activities are a concern this may be important to downstream success.

Our BirA enzyme is E. coli wild-type, encoded by the birA gene, and purified to greater than 99% purity by traditional methods. Other names for this enzyme include: biotin ligase; biotin operon repressor protein; birA; biotin holoenzyme synthetase; biotin-[acetyl-CoA carboxylase] synthetase; biotin-[acetyl-CoA-carboxylase] ligase; biotin-[acetyl-CoA carboxylase] synthetase; acetyl CoA holocarboxylase synthetase; acetyl CoA holocarboxylase synthetase; biotin:apocarboxylase ligase; biotin holoenzyme synthetase; HCS.

Avidity研发和销售用于分子连接的分子亲和工具，其的AviTag™技术采用针对性很强的一个独特的15氨基酸肽，用来自大肠杆菌的生物素连接酶（BirA）标签上的单一的生物素酶的共轭。   Avidity develops and sells molecular affinity tools for connecting molecules. Our patented AviTag™ technology employs a highly targeted enzymatic conjugation of a single biotin on a unique 15 amino acid peptide tag using the biotin ligase （BirA） from E. coli. AviTag™ sequence is GLNDIFEAQKIEWHE.  Oriented on streptavidin-coated surfaces, this creates an ideal presentation for molecular binding interactions. 公司：www.avidity.com   产品编号 产品名称 规格 价格（元） 生产 AbC Anti-C-terminus AviTagTM Antibody 100 ul ￥3,600.00 Avidity AbCA Anti-C-terminus AviTagTM –conjugated Agarose Resin 2 ml ￥4,500.00 Avidity AVB100 Efficient Biotinylation E. coli K12 Strain AVB100 1 vial ￥4,500.00 Avidity AVB101 Efficient Biotinylation E. coli B Strain AVB101 1 vial ￥4,500.00 Avidity AVB99 Efficient Biotinylation E.coli K12 Strain AVB99 1 vial ￥4,500.00 Avidity BIO200 Biotin Solution 1.5 ml ￥810.00 Avidity BIO500 Biotin Solution 10 ml ￥1,620.00 Avidity BIP300 Positive Control Plasmid 2 ug ￥2,700.00 Avidity BIRA500 BirA Biotin-Protein Ligase 40 ug ￥4,500.00 Avidity BIS300 Positive Control Protein Substrate Kit 1 kit ￥2,700.00 Avidity Bulk BIRA Bulk BirA Biotin-Protein Ligase 300 ug ￥17,640.00 Avidity CFT100 Mammalian Cell-Free Translation System: AvidExpressTM 1 kit ￥3,150.00 Avidity CTZ-1 Native Coelenterazine Free Base 1 mg ￥3,600.00 Avidity CVB101 Chemically Competent E.coli B Strain AVB101 10 vials ￥3,150.00 Avidity EVB100 Electrocompetent E. coli K12 Strain AVB100 10 vials ￥3,150.00 Avidity EVB101 Electrocompetent E. coli B Strain AVB101 10 vials ￥3,150.00 Avidity FASP Sequence Primer-Forward 200 pmol ￥1,710.00 Avidity Gluc-10 Biotinylated Gaussia princeps Luciferase 10 ug ￥1,350.00 Avidity Gluc-100 Biotinylated Gaussia princeps Luciferase 100 ug ￥6,300.00 Avidity pAC4 Biotin AviTagTM Vectors（C-terminal） 10 ug ￥2,970.00 Avidity pAC5 Biotin AviTagTM Vectors（C-terminal） 10 ug ￥2,970.00 Avidity pAC6 Biotin AviTagTM Vectors（C-terminal） 10 ug ￥2,970.00 Avidity pACS Biotin AviTagTM Vectors（C-terminal） 1 kit ￥8,100.00 Avidity pAN4 Biotin AviTagTM Vectors（N-terminal） 10 ug ￥2,970.00 Avidity pAN5 Biotin AviTagTM Vectors（N-terminal） 10 ug ￥2,970.00 Avidity pAN6 Biotin AviTagTM Vectors（N-terminal） 10 ug ￥2,970.00 Avidity pANS Biotin AviTagTM Vectors（N-terminal） 1 kit ￥8,100.00 Avidity RASP Sequence Primer-Reverse 200 pmol ￥1,710.00 Avidity rrGFP-100 Biotinylated Renilla reniformis Green Fluorescent Protein 100 ug ￥5,400.00 Avidity SA-Gluc Streptavidin-conjugated Gaussia princeps Luciferase 50 ug ￥4,500.00 Avidity SA-rrGFP 1.0 Streptavidin-conjugated Renilla reniformis Green Fluorescent Protein 160 ug ￥3,600.00 Avidity