The DetectX^® Triiodothyronine (T₃) Enzyme Immunoassay kit is designed to quantitatively measure T₃ present in urine, extracted serum and plasma, extracted dried fecal samples, and tissue culture media samples. This kit measures total T₃ in extracted serum and plasma and in extracted fecal samples.

A T₃ stock solution is provided to generate standard curves for the assay. Standards or diluted samples are pipetted into a coated microtiter plate. A T₃-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a sheep antibody to T₃ to each well. After a two hour incubation the plate is washed and substrate is added. The substrate reacts with the bound T₃-peroxidase conjugate. After a 30 minute incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450nm.

Arbor Assays的员工在仅用于研究目的和FDA体外诊断市场的设计，开发，构建和制造测定法方面拥有超过50年的综合经验。我们绝不妥协质量。在整个网站上，您会在几个页面上看到“ N-Cal™”字样。这是指将标准品校准至WHO或美国国家标准技术研究院（NIST）参考材料的试剂盒。任何套件都仅与参考的标准一样好。