The DetectX^® Testosterone ELISA Kit uses a specifically generated antibody to measure testosterone and its metabolites in urine and fecal samples, or in extracted serum and plasma. This kit is not recommended for serum, plasma, or saliva samples without extraction. The kit will quantitatively measure Testosterone present in reconstituted buffer samples and tissue culture media samples. A testosterone standard is provided to generate a standard curve for the assay. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A testosterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to testosterone to each well. After a 2 hour incubation the plate is washed and substrate is added. The substrate reacts with the bound testosterone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450nm wavelength.

Arbor Assays的员工在仅用于研究目的和FDA体外诊断市场的设计，开发，构建和制造测定法方面拥有超过50年的综合经验。我们绝不妥协质量。在整个网站上，您会在几个页面上看到“ N-Cal™”字样。这是指将标准品校准至WHO或美国国家标准技术研究院（NIST）参考材料的试剂盒。任何套件都仅与参考的标准一样好。