The DetectX^® 13,14,Dihydro-15-keto-Prostaglandin F_2α (PGFM) ELSIA Kit measures PGFM present in non-invasively collected fecal and urine samples. The assay will also measure PGFM in plasma, serum and TCM samples. The kit has been developed for urine and extracted fecal samples and has been validated with Iberian lynx and other felid samples. After an hour incubation the plate is washed and substrate is added. The substrate reacts with the bound PGFM-peroxidase conjugate and the reaction is read at 450nm wavelength.

PGFM has been suggested as a useful non-invasive pregnancy marker both in urine and fecal samples. It has been shown to be a precise, practical method for this application in these matrices for a number of species. Parallel courses were obtained when comparing urinary and fecal PGFM in a variety of felids and other species, and only a simple dilution of fecal extracts is necessary prior to analyses. Fecal PGFM analyses may allow pregnancy diagnosis in captive and free-ranging felids. Recent evidence has suggested that PGFM may also be a useful pregnancy marker in some other non-felid species.

Arbor Assays的员工在仅用于研究目的和FDA体外诊断市场的设计，开发，构建和制造测定法方面拥有超过50年的综合经验。我们绝不妥协质量。在整个网站上，您会在几个页面上看到“ N-Cal™”字样。这是指将标准品校准至WHO或美国国家标准技术研究院（NIST）参考材料的试剂盒。任何套件都仅与参考的标准一样好。