The DetectX^® Prostaglandin E₂ (PGE₂) Multi-Format ELISA Kit is designed to quantitatively measure PGE₂ present in serum, plasma, urine, tissues, saliva and culture media samples. This one ELISA kit allows all samples to be measured with just one kit.  The monoclonal antibody has super-fast kinetics and shows the same binding characteristics at 2 or 16 hour incubation. The kit allows samples as low as 2 pg/mL to be measured in less than 3 hours. It exhibits excellent cross reactivity and low sample requirements.

A PGE₂ standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. A PGE₂-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a monoclonal antibody to PGE₂. After either an incubation the plate is washed and substrate is added. The substrate reacts with the bound PGE₂-peroxidase conjugate. After a 30 minute incubation the intensity of the generated color is read at 450nm.

The new PGE₂ monoclonal (3H10) developed to use in the new PGE₂ EIA kits has some truly amazing properties:

• Super Fast Kinetics – Same Assay Range at 2 or 16 hours
• Incredible Sensitivity – Lowest Standard < 2 pg/mL
• Superior Cross Reactivity to Other Eicosanoids
• Super Low Sample Volume Requirements
• Choice of Incubation Time, Sensitivity, and Sample Volume in a Single Kit

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