ARBOR ASSAYS/Glutathione Reductase (GR) Fluorescent Activity Kit/K009-F1/F1

价格
¥5500.00
货号:K009-F1
浏览量:127
品牌:ARBOR ASSAYS
服务
全国联保
正品保证
正规发票
签订合同
商品描述
  • MostSensitive
  • MultiSpecies
  • USE-MeasureGRin20MInutesorKinetically
  • SAMPLE-Lysate,RBCs,SerumandPlasma
  • SAMPLES/KIT-41inDuplicate
  • SENSITIVE-9µU/mL
  • STABILITY-Stable4˚CLiquidReagents

ViewPublications

TheDetectX®GlutathioneReductase(GR)FluorescentActivityKitdeterminesGRactivitybytheamountofGSHgeneratedfromthereductionofGSSGwithanon-fluorescentmolecule,ThioStar®,thatcovalentlybindsthefreethiolgrouponGSHtoyieldahighlyfluorescentproduct.ThemostwidelyusedproceduretomeasureGRistomonitortheoxidationofNADPHasadecreaseinabsorbanceat340nm.HowevermanyBIOLOGicalmoleculesabsorblightat340nm,plusthedetectionsystemgivesverylowODreADIngs.TheARBORASSAYS’DetectX®GlutathioneReductaseFluorescentActivitykitovercomestheseproblemsofmeasuringGRactivitybythedirectdetectionoftheGSHformedfromoxidizedglutathione.

Glutathionereductase(GR)playsanindirectbutessentialroleinthepreventionofoxidativedamagewithinthecellbyhelpingtomaintainappropriatelevelsofintracellularglutathione(GSH).GSH,inconjuctionwiththeenzymeglutathioneperoxidase(GP),istheactingreductantresponsIBLeforminimizingharmfulhydrogenperoxidecellularlevels.TheregenerationofGSHiscatalyzedbyGR.GRisanubiquitous100-120kDadimericflavoproteinthatcatalyzesthereductionofoxidizedglutathione(GSSG)toreducedglutathione,usingß-nicotinamidedinucleotidephosphate(NADPH)asthehydrogendonor.MoleculessuchasNADPHactashydridedonorsinavarietyofenzymaticprocesses.NADPHhasbeensuggestedtoalsoactasanindirectlyoperatingantioxidant,givenitsroleinthere-reductionofGSSGtoGSHandthusmaintainingtheantioxidativepowerofglutathione.

Glutathione Reductase Activity Standard Curve

DataReductionTemplatebyMyAssays

Arbor Assays的员工在仅用于研究目的和FDA体外诊断市场的设计,开发,构建和制造测定法方面拥有超过50年的综合经验。我们绝不妥协质量。在整个网站上,您会在几个页面上看到“ N-Cal™”字样。这是指将标准品校准至WHO或美国国家标准技术研究院(NIST)参考材料的试剂盒。任何套件都仅与参考的标准一样好。