The DetectX^® Estrone ELISA Kit is designed to quantitatively measure estrone present in extracted dried fecal samples, urine and tissue culture media samples. The kit is unique as it measures both non-conjugated estrone, estrone-3-sulfate and estrone 3-glucuronide in urine and fecal samples with almost equal affinity, allowing for non-invasive testing of this steroid. An estrone standard is provided to generate a standard curve for the assay. Standards or diluted samples are pipetted into a clear microtiter plate. An estrone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to estrone to each well. After a 2 hour incubation the plate is washed and substrate is added. The substrate reacts with the bound estrone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is read at 450nm.

Arbor Assays的员工在仅用于研究目的和FDA体外诊断市场的设计，开发，构建和制造测定法方面拥有超过50年的综合经验。我们绝不妥协质量。在整个网站上，您会在几个页面上看到“ N-Cal™”字样。这是指将标准品校准至WHO或美国国家标准技术研究院（NIST）参考材料的试剂盒。任何套件都仅与参考的标准一样好。