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Product Name	12-HETE(12-Hydroxyeicosatetraenoic Acid) ELISA Kit
Species	General
Description	This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 12-HETE. During the reaction, 12-HETE in the sample or standard competes with a fixed amount of 12-HETE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 12-HETE. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 12-HETE in the samples is then determined by comparing the OD of the samples to the standard curve.
Synonyms	12-Hydroxyeicosatetraenoic Acid
Method	Competitive ELISA
Detection Range	0.78-50ng/mL
Sensitivity	0.63ng/mL
Sample Types	Serum, Plasma, Cell supernatant
Intended Use	For research use only, not for use in diagnostic procedures.

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