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Product Name	AAV-6 Titration ELISA
Description	Unique microtiter plate enzyme immunoassay for the quantitation of intact AAV6 wt virions, AAV6 recombinant virions or assembled and intact empty capsids of human Adeno-associated Virus 6. The capture-antibody detects a conformational epitope not present on unassembled capsid proteins.
Background	Adeno-associated viruses (AAV) are non-pathogenic ssDNA viruses, which are a subject of intense studies as viral vectors for gene therapy. The virus transduces a variety of dividing and non- dividing cells showing long-term gene expression with low cellular immune response. AAV has been used in several clinical trials (e.g. FIX, CFTR, Parkinson’s, Canavan disease) showing no serious vector-related adverse effects. Methods for the characterization of AAV preparations currently include titration ELISA, real-time PCR, DNA dot blot, determination of transducing units, infectious center assay, SDS-PAGE or electron microscopy. Immunotitration by ARP's AAV6 Titration ELISA offers a fast, sensitive and reproducible method for titration of intact AAV6 wt virions, AAV6 recombinant virions or assembled and intact empty AAV6 capsids.
Principle	The assay is based on the sandwich ELISA technique. A monoclonal antibody specific for a conformational epitope on assembled AAV6 capsids (ADK6) is coated onto strips of a microtiter plate and is used to capture AAV6 particles from the specimen. Captured AAV particles are detected in two steps. First, a biotin-conjugated monoclonal antibody to AAV6 (ADK6) is bound to the immune complex. Second, a streptavidin peroxidase conjugate reacts with the biotin molecules. Addition of substrate solution results in a color reaction, which is proportional to the amount of specifically bound viral particles. The absorbance is measured photometrically at 450 nm. The provided Kit Control contains an AAV6 particle preparation of empty capsids. Two-fold serial dilutions of the material result in a typical titration curve. The curve allows the quantitative determination of samples of an unknown particle titer.
Storage	2-8C
Intended Use	Limited Use Label License: Research Use OnlyProduct is exclusively licensed to PROGEN Biotechnik GmbH. The use of these products for the development, manufacturing and sale of secondary products/derivatives which are based on the purchased products and/or which include the purchased product require a royalty based sub-license agreement.

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