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Product Name	AAV-1 Titration ELISA
Description	Enzyme Immunoassay for the Quantitative Determination of AAV Serotype 1 Particles in Cell Culture Supernatants and Purified Virus Preparations.
Background	Adeno-associated virus (AAV) is a ssDNA virus that is a topic of intense study in gene therapy. The different serotypes transduce a wide variety of dividing and non-dividing cells showing long-term gene expression. AAV 1 has been used in several studies showing superior infection of muscle, liver and vascular endothelium when compared to other AAV serotypes. Until now no serious vector-related adverse effects have been observed. Methods for the characterisation of AAV preparations currently include titration ELISA, real-time PCR, DNA dot blot, determination of transducing units, infectious center assay, SDS- PAGE or electron microscopy.Immunotitration by ARP's AAV 1 Titration ELISA offers a fast, sensitive and reproducible method for titration of intact AAV 1 wt virions, AAV 1 recombinant virions or assembled and intact empty AAV 1 capsids.
Principle	The assay is based on the sandwich ELISA technique. A monoclonal antibody specific for a conformational epitope on assembled AAV 1 capsids is coated onto microtiter strips and is used to capture AAV 1 particles from the specimen. Captured AAV 1 particles are detected in two steps. First a biotin conjugated monoclonal antibody to AAV 1 is bound to the immune complex. In the second step streptavidin peroxidase conjugate reacts with the biotin molecules. Addition of substrate solution results in a color reaction which is proportional to the amount of specifically bound viral particles. The absorbance is measured photometrically at 450 nm. The kit control provided contains an AAV 1 particle preparation of intact capsids. It shows a typical titration curve when used in dilutions of steps of two (Fig. 1). It allows quantitative determination of samples of an unknown particle titer (immunological titer) and the calibration of an inhouse AAV 1 preparation (e.g. infectious titer, transducing units, DNA dot blot titer, Table 1).
Storage	2-8C
Intended Use	Limited Use Label License: Research Use OnlyProduct is exclusively licensed to PROGEN Biotechnik GmbH. The use of these products for the development, manufacturing and sale of secondary products/derivatives which are based on the purchased products and/or which include the purchased product require a royalty based sub-license agreement.

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