| Clone | ITYV |
| Isotype | IgG1 |
| Product Type | Single-Color Reagent |
| Units | 100 µg |
| Host | Mouse |
| Species reactivity | Human |
| Application | Flow Cytometry |
BackgroundIdentification of human receptor for Interleukin-2 (IL-2R) expressing the 55,000 M.W. surface antigen.
Synonyms: CD25*
Source
Immunogen: CD25=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells of BALB/c mice immunized with PHA-activated human T-lymphocytes.
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
ApplicationsPBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Functional Analysis: Flow Cytometry Staining
StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: Products are stable for one year from purchase when stored properly
Shipping Conditions: Room Temperature
CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
References1. A Monoclonal Antibody (Anti-Tac) Reactive with Activated and Functionally Mature Human T Cells. Uchiyama T., Broder S., Waldmann TA, 1981 J. Immunol.126,1393. 2. Direct Demonstration of the Identity of T Cell Growth Factor Binding Protein and the Tac Antigen. Robb RJ, Greene WC, J. Exp. Med. 1983,158;1332. 3. Patients with HIV infection have a reduced proportion of lymphocytes expressing the IL2 receptor p55 chain (TAC, CD25). Zola H., Koh L.Y., Mantzioris B.X., Rhodes D., Clin. Immunol. Immunopathol. 1991 Ap:59(1):16-25. 4. Spontaneous lymphocyte proliferation in HTLV-I/II infection reflects preferential activation of CD8 and CD16/56 cell subsets. Prince H.E., Weber D.M., Jensen E.R., Clin. Immunol. Immunopathol. 1991, May;58(3):419-30. 5. Defective clonogenic potential of CD8+ T lymphocytes in patients with AIDS. Expansion in vivo of a nonclonogenic CD3+ CD8+ DR+ CD25- T cell population. Pantaleo G., Keonig S., Baseler M., Lane H.C., Fauci A.S., J. Immunol. 1990 Mar ; 144(5):1696-704.
Protein Reference(s)
Database Name: UniProt
Accession number: P01589
Safety Datasheet(s) for this product:NM_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium Azide Noridic-MUbio.pdf
