| Clone | AX.14 |
| Isotype | IgG1 |
| Product Type | Single-Color Reagent |
| Units | 100 Tests |
| Host | Mouse |
| Species reactivity | Human |
| Application | Flow Cytometry |
BackgroundThe CD103 antigen is a 160-130-105 kDa molecule which is expressed on mucosa-associated T lymphocytes and activated cellsand on subset of TGF beta -1 cells. This human intra epithelial lymphocyte marker is theE integrin chain associated with the b7 subunit. CD103 antibody stains a few bone marrow cells and, rarely,peripheral blood lymphocytes.CD103 is expressed by 0.5 to 2 % of resting lymphocytes in Peripheral blood and lymphoid organs.
Synonyms: CD103 FITC
Product
Product Form: FITC
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
Purification Method: Protein A/G Chromatography
Concentration: Titered for flow cytometry
ApplicationsPBMC: Add 10 µl of MAB/106 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2° to 8° C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS andanalyze.WHOLE BLOOD:Add 10 µl of MAB/100 µl of whole blood.Mix gently and incubate for 15 minutes at room temperature (20° C).Lyse the whole blood. Wash once with PBS and analyze or fix with0.5% v/v of paraformaldehyde in PBS and analyze.See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN:(APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters.Laser excitation is at 633 nm with a Helium Neon(HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser.Peak fluorescence emission is at 660 nm.
Functional Analysis: Flow Cytometry Staining
StorageProduct should be stored at 4-8ºC. DO NOT FREEZE
Product Stability: Reagents are stable for the period shown on the vial label when stored properly
Shipping Conditions: Room Temperature
CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
References1. Cepek K., Wong D. Brenner M., and Springer T., Leukocyte Typing V val. G p 1666-67 (1995).
Protein Reference(s)
Database Name: UniProt
Accession number: P38570
Safety Datasheet(s) for this product:NM_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium Azide Noridic-MUbio.pdf
