Nordicmubio/CD31 (PECAM-1), human/CD31-1

价格
面议
货号:CD31-1
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品牌:Nordic
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商品描述
CD31 (PECAM-1), humanCatalogue number: CD31-1

CloneJC70A
IsotypeIgG1
Product Type Primary Antibodies
Units1 ml
HostMouse
Application Flow CytometryImmunohistochemistry (frozen)Immunohistochemistry (paraffin)

BackgroundThe antibody JC70A primarily is used for the immunohistological detection of endothelial cells in Formalin-fixed tissues. Positive reactions are expected as well in normal as in transformed tissue. The antibody frequently is used for evaluation of angiogenesis in proliferating tumours (e.g. in mamma carcinoma, colorectal carcinoma and lung cancer). Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1) or CD31 belongs to a group of transmembrane glycoproteins of the immunoglobulin super-family of adhesion molecules. It is exprimed predominantly by megakaryocytes, platelets, granulocytes, B-cell precursors and endothelial cells. It plays an important role in adhesion of leucocytes to blood vessel endothelia and for the cell migration through the vessel walls. Human CD31, Formalin-resistant epitope on a 130 kDa glycoprotein chain, reactivity confirmed on V. Internat. Workshop on Leucocyte Typing (Boston 1993).

Source

Immunogen: Spleen cell membrane preparation of hairy cell leukaemia affected patient

ProductAntibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGOArt .No. PU002.

Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGOArt .No. PU002.

Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Specificity

Species Reactivity: Human

ApplicationsIHC(C, P), FACS

Incubation Time: 60 min at RT

Working Concentration: (liquid conc.) 1:10

Pre-Treatment: Pre-treatment of formalin-fixed, paraffin-embedded tissues at high temperature with citrate buffer (art. no. DE000) or better with TRIS-buffered EDTA (art. no. DE006).

Positive Control: Tonsil

Storage2-8°C

Caution*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

References1. Parums D.V., Cordell J.L., Micklem K., Heryet A.R., Gatter K.C., and Mason D.Y. (1990) JC70: a new monoclonal antibody that detects vascular endothelium associated antigen on routinely processed tissue sections. J. Clin. Pathol. 43; 752-757. 2. Newman P.J., and Paddock C. (1995) CD31 cluster workshop report. In: Schlossman S.F. et al. Leucocyte Typing V. White Cell Differentiation Antigens. Oxford University Press, Oxford, N.Y., Tokyo pp 1259-1260. 3. Liao F., Huynh H.K., Eiora A., Greene T., Polizzi E., Muller W.A. (1995) Migration of monocytes across endothelium and passage through extracellular matrix involve separate molecular domains of PECAM 1. J. Exp. Med. 182; 1337-1343. 4. Buckley C.D., Doyannas R., Newton J.P., Blystone S.D., Brown E.J., Watt S.M., et al. (1996) Identification of alpha v and beta 3 as a heterotypic ligand for CD31/PECAM-1. J. Cell Sci. 109; 437-445.

Nordic的纯化的牛IgG可以在各种免疫测定(包括免疫扩散,免疫电泳,ELISA,Western印迹,斑点免疫结合测定(DIBA),血细胞凝集和细胞结合测定)中用作参考抗原,校准物,包被蛋白和封闭剂。该产品仅用于研究目的,并且用于体外测试,不适用于涉及人或动物的诊断或治疗程序。它可能包含有害成分。请参阅安全数据表(SDS)了解更多信息和正确的处理程序。根据当地法规处理产品残留物。此数据表的准确性尽可能合理,但Nordic-MUbio对本信息中的任何准确性或遗漏概不负责。