Mouse anti Human Calpain I, II (small subunit), conjugated with agaroseCatalogue number: X1011

Clone	28F3
Isotype	IgG1
Product Type	Monoclonal Antibody
Units	1 ml
Host	Mouse
Species reactivity	Human
Application	ELISAImmunoprecipitation

BackgroundThe calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and µ-calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar (mM) levels of calcium, µ-calpain is active at micromolar (µM) concentrationsofCa ++. Inadditiontotheubiquitouslyexpressedm-and µcalpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.

Synonyms: Mouse anti calpain I, II small subunit, p30, agarose conjugate

Source

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with human calpain with mouse myeloma.

Product

Product Form: Agarose

Formulation: Provided as a concentration of 1 mg/ml in 0.02M sodiumphosphate, pH 7.5, 0.15M sodium chloride, 50% glycerol, 3mM sodium azide.

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

ApplicationsApplication: Use at 0.5-1 ug/ml for ELISA and western blot, 1-2ug/ml for immunoprecipitation.

Functional Analysis: Western Blotting

StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Antibodies are stable for one year from purchase if stored frozen

Shipping Conditions: Room Temperature

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References1. Adamec, E., et al. 'Calpain I activation in rat hippocampal neurons in culture is NMDA receptor selective and not essential for excitotoxic cell death.' Brain Res. Mol. Brain Res. 1998, 54, 35-482. Guttmann, R.P. et al. 'Oxidation inhibits substrate proteolysis by calpain I but not autolysis.' J. Biol. Chem. 1997, 272, 2005-2012

Protein Reference(s)

Database Name: UniProt

Accession number: P17655

Species Accession: Human

Safety Datasheet(s) for this product:NM_GlycerolNM_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium Azide Noridic-MUbio.pdf/wp-content/uploads/SDS/SDS Antibodies with glycerol Nordic-MUbio.pdf

Nordic的纯化的牛IgG可以在各种免疫测定（包括免疫扩散，免疫电泳，ELISA，Western印迹，斑点免疫结合测定（DIBA），血细胞凝集和细胞结合测定）中用作参考抗原，校准物，包被蛋白和封闭剂。该产品仅用于研究目的，并且用于体外测试，不适用于涉及人或动物的诊断或治疗程序。它可能包含有害成分。请参阅安全数据表（SDS）了解更多信息和正确的处理程序。根据当地法规处理产品残留物。此数据表的准确性尽可能合理，但Nordic-MUbio对本信息中的任何准确性或遗漏概不负责。