| Clone | FG4R |
| Isotype | IgG2b |
| ProductType | MonoclonalAntibody |
| Units | 100µg |
| Host | Mouse |
| Application | DotblotELISAImmunoprecipitationWesternBlotting |
BackgroundEpitopetagsareausefulwaytoidentifyprotiensinavarietyofcelltypes,studythetopologyofproteinsandproteincomplexes,identifyassociatedproteins,andcharacterizenewlyidentified,lowabundanceorpoorlyimmunogenicproteinswhenproteinspecificantibodiesarenotavailable.TaggingwithExalpha"sDYKDDDDKFLAGantibodymaybedoneattheN-terminus,N-terminusprecededbyamethionineresidue,C-terminus,andininternalpositionsofthetargetprotein.Thesmallsizeofthisepitopetaganditshighhydrophilicitytendtodecreasethepossibilityofinterferencewithproteinexpression,proteolyticmaturation,antigenicityandfunction.Theenterokinasecleavagesiteallowsittobecompletelyremovedfromthepurifiedfusionproteins.ReactswithFLAGtaggedfusionproteins.
Source
Immunogen:DYKDDDDK(FLAG)syntheticpeptideconjugatedtoKLH
Product
ProductForm:Unconjugated
Formulation:10mMPBS,pH7.2,with0.05%SodiumAzide
PurificationMethod:ProteinA/GChromatography
Concentration:Seevialforconcentration
ApplicationsWB(withECL):1:1000-3000dilution(incubateforonehouratroomtemperature)IS:1:500-2000dilutionForbestresultswithotherassays(e.g.:Dot,ELISA,IP,etc),pleasedetermineoptimalworkingdilutionbytitrationtest
FunctionalAnalysis:WesternBlotting
PositiveControl:RecognizestheN-terminal,C-terminalorinternalDYKDDDDK-taggedfusionproteinsRecognizes1-5ngofFLAGtaggedproteinsinwBwithECLdetection
StorageProductshouldbestoredat-20ºC.Aliquottoavoidfreeze/thawcycles
ProductStABIlity:Productsarestableforoneyearfrompurchasewhenstoredproperly
ShippingConditions:Shipatambienttemperature,freezeuponarrival
CautionThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Itmaycontainhazardousingredients. PleaserefertotheSafetyDataSheets(SDS)foradditionalinformationandproperhandlingprocedures.Disposeproductremaindersaccordingtolocalregulations.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-Mubioacceptsnoliabilityforanyinaccuraciesoromissionsinthisinformation.
SafetyDatasheet(s)forthisproduct:NM_SodiumAzide/wp-content/uploads/SDS/AntibodySDSwithSodiumAzideNoridic-MUbio.pdf
Nordic的纯化的牛IgG可以在各种免疫测定（包括免疫扩散，免疫电泳，ELISA，Western印迹，斑点免疫结合测定（DIBA），血细胞凝集和细胞结合测定）中用作参考抗原，校准物，包被蛋白和封闭剂。该产品仅用于研究目的，并且用于体外测试，不适用于涉及人或动物的诊断或治疗程序。它可能包含有害成分。请参阅安全数据表（SDS）了解更多信息和正确的处理程序。根据当地法规处理产品残留物。此数据表的准确性尽可能合理，但Nordic-MUbio对本信息中的任何准确性或遗漏概不负责。
