Unmasking fluid E, TRIS-buffered EDTA solution, pH 9Catalogue number: DE006-250

Product Type	Buffers and Reagents
Units	250 ml
Application	Immunohistochemistry (paraffin)

BackgroundAntigen unmasking based on citrate buffer are most frequently used for unmasking hidden epitopes. However for the detection of some antigens or the simultaneous detection of two or more antigens more sophisticated buffers may be required. Unmasking fluid E on Tris/EDTA basis has been used for a number of antigens and is specially useful for the detection of a number of CD markers, P504S, p63 and HMW cytokeratins etc. Aldehyde fixation (Formaldehyde fixation) of tissues generally causes cross-linkage of protein chains and results in masking of antigens present in the treated tissue. This makes the antigen epitopes undetectable for many antibodies applied for immunohistochemistry. Mainly monoclonal antibodies, but also mono-specific polyclonal antibodies will react with reduced binding or no binding at all. Since Formaldehyde fixation is the most important fixation method in routine histology, high temperature unmasking methods for immunohistochemistry, as developed during recent years, are very important tools for cell and tissue research and pathology.

Product250 ml 10-times concentrated solution

Purification Method: 250 ml 10-times concentrated solution

ApplicationsIHC(P)

Working Concentration: (10 x concentrated solution) 1:10

Positive Control: CD1a, CD2, CD4, CD9, CD22, CD30, CD31, CD40, cdk2, MAGE-1, p63, P504S (AMACR), Retinoblastoma, Tenascin, Tyrosinase, TTF-1, HMW-Cytokeratin, PIN-Cocktails 1 and 2*

Storage2-25°C

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References

Nordic的纯化的牛IgG可以在各种免疫测定（包括免疫扩散，免疫电泳，ELISA，Western印迹，斑点免疫结合测定（DIBA），血细胞凝集和细胞结合测定）中用作参考抗原，校准物，包被蛋白和封闭剂。该产品仅用于研究目的，并且用于体外测试，不适用于涉及人或动物的诊断或治疗程序。它可能包含有害成分。请参阅安全数据表（SDS）了解更多信息和正确的处理程序。根据当地法规处理产品残留物。此数据表的准确性尽可能合理，但Nordic-MUbio对本信息中的任何准确性或遗漏概不负责。