| Isotype | Ig |
| Product Type | Antigen Immunoaffinity Purified Polyclonal |
| Units | 100 µg |
| Host | Rabbit |
| Species reactivity | Human |
| Application | Immunohistochemistry (paraffin) |
BackgroundDNA Topoisomerase 1 releases the supercoiling and torsional tension of DNA introduced during DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. It introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.
Source
Immunogen: Synthetic peptide made to human DNA Topoisomerase 1
Product
Product Form: Affinity Purified
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Antigen Immunoaffinity Purification
Concentration: See vial for concentration
ApplicationsAntibody can be used for immunohistochemistry at 5-10 µg/ml on paraffin-embedded tissues.Optimal concentration should be evaluated by serial dilutions.
Positive Control: Found in Endothelial cells.
StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: Products are stable for one year from purchase when stored properly
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
References1. Oddou, P., et al. 'Monoclonal antibodies neutralizing mammalian DNA topoisomerase I activity.' Eur. J. Biochem. 1988, 177, 523-5292. Redinbo, M.R., et al. 'Novel insights into catalytic mechanism from a crystal structure of human topoisomerase I in complex with DNA.' Biochemistry 2000, 39, 6832-68403. Eisenreich, A., et al. 'Cdc2-like kinases and DNA topoisomerase I regulate alternative splicing of tissue factor in human endothelial cells.' Circ. Res. 2009, 104, 589-599
Protein Reference(s)
Database Name: UniProt
Accession number: P11387
Species Accession: Human
Safety Datasheet(s) for this product:NM_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium Azide Noridic-MUbio.pdf
