Background:

HumanGFAP-δ,alsotermedGFAP-ε,isanalternativesplicevariantoftheGFAPprotein.GFAPproteinsareselectivelylocatedinastrocytesandrepresentthemajorconstituentofastrocyticintermediatefilaments.GFAPexpressionlevelsarehighlyvariableduringdevelopmentofthecentralnervoussystem.GFAP-δdiffersfromthepredominantspliceformofGFAP,GFAP-α,initsC-terminalsequenceinwhichtheoriginalaminoacidsequence,encodedbyexons8and9,arereplacedbyauniqueandconservedtailof42aminoacidsencodedbyexon7+andcalledtheGFAP-δtaildomain.ThisGFAP-δtaildomainisabletobindpresenilinproteinsofwhichmutatedformsareacauseofAlzheimerdisease.GFAP-δdoeshowevernotdiscriminatebetweenAlzheimerandcontrolfissue.UpregulationofGFAP-δhasanegativeeffectonGFAPfilamentformation.IncontrasttoGFAP-α,GFAP-δisnotupregulatedinastrogliosis.

Source:

GFAP-δisarabbitpolyclonalantiserumderivedbyimmunizationwiththepeptideQAHQIVNGTPPARG,representingthelast14C-terminalaminoacidsoftheGFAP-δprotein.

Product:

Eachvialcontains100μlrabbitpolyclonalserumcontaining0.09%sodiumazide.

Applications:

GFAP-δissuitableforimmunohistochemistryonparaffinsectionsandWesternblotting.Optimalantibodydilutionshouldbedeterminedbytitration;recommendedrangeis1:100—1:500forimmunohistochemistryand1:50—1:1000forWesternblotting.

Specificity:

GFAP-δstainstheastrocyteslocatedinthesubpialzoneofthecerebralcortex,thesubventricularzoneofthehippocampusand,mostintensely,aribbonofastrocytesfollowingtheependymallayerofthecerebralventricles.

Storage:

Storeat4°C,orinsmallaliquotsat-20°C.

References:

1.Roelofs,R.F.,Fischer,D.F.,Houtman,S.H.,Sluijs,J.A,vanHaren,W.,vanLeeuwen,F.
W.,andlVbl,E.M.(2005).Adulthumansubventricular,subgranular,andsubpialzones
containastrocyteswithaspecializedintermediatefilamentCytoskeleton,GLIA52,289-300.
2.NielsenAL.,Holm,l.E.,Johansen,M,Bonven,B.,Jorgensen,P.,Jorgensen,A.L.,
(2002).Anewsplicevariantofglialfibrillaryacidicprotein,GFAPepsilon,interactswiththe
presenilinproteins.J.Biol.Chem.277,29983-91.

Caution:

ThisproductisintendedFORRESEARCHUSEONLYandFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisproductcontainssodiumazide.Topreventformationoftoxicvapors,donotmixwithstrongacidicsolutions.Topreventformationofpotentiallyexplosivemetallicazidesinmetalplumbing,alwayswashintodrainwithcopiousquantitiesofwater.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-MubioBVacceptsnoIiABIlityforanyinaccuraciesoromissionsinthisinformation. Nordic的纯化的牛IgG可以在各种免疫测定（包括免疫扩散，免疫电泳，ELISA，Western印迹，斑点免疫结合测定（DIBA），血细胞凝集和细胞结合测定）中用作参考抗原，校准物，包被蛋白和封闭剂。该产品仅用于研究目的，并且用于体外测试，不适用于涉及人或动物的诊断或治疗程序。它可能包含有害成分。请参阅安全数据表（SDS）了解更多信息和正确的处理程序。根据当地法规处理产品残留物。此数据表的准确性尽可能合理，但Nordic-MUbio对本信息中的任何准确性或遗漏概不负责。