Background:

ThereactivityoftheantiserumisdirectedtotheFcsubunitoftheIgAmoleculewhichexpressesstrictisotypic(class)specificity.Itdoesnotreactwithanynon-Igproteinindogserum,astestedbyimmunoelectrophoresisanddoublerADIalimmunodiffusion.InimmunocytochemicalandimmunohistochemicalstainingofIgAatthecellularandsubcellularlevelofappropriatelytreatedcellandtissuesubstrates;todemonstratecirculatingIgAantibodiesinSEROdiagnosticmicroBIOLOGyandautoimmunediseases;toidentifyaspecificantigenusingareferenceantibodyofdogoriginknowntobeoftheIgAisotypeinthemiddlelayeroftheindirecttestprocedure;innon-isotopicassaymethodology(e.g.ELISA)tomeasureIgAindogserumorotherbodyfluids.Asasecondstepanavidinorstreptavidinconjugateoftheuser’schoicehastobeused.AntiseratoIgAdonotdiscriminatebetweenserumIgA(monomericanddimeric)andhighermolecularformssuchassecretoryIgA.Thisimmunoconjugateisnotpre-diluted.Theoptimumworkingdilutionofeachconjugateshouldbeestablishedbytitrationbeforebeingused.Excesslabelledantibodymustbeavoidedbecauseitmaycausehighunspecificbackgroundstainingandinterferewiththespecificsignal.

Source:

PurifiednormalIgAisolatedfrompooleddogserum.Freund’scompleteadjuvantisusedinthefirststepoftheimmunizationprocedure.

Product:

Biotin-coupledpurifiedhyperimmunegoatIgGlyophilizedfromasolutioninphosphatebufferedsaline(PBS,pH7.2).Nopreservativeadded,asitmayinterferewiththeantibodyactivity.IgGproteinconcentration10mg/ml.Biotin/IgGproteinmolarratio(B/P)isapproximately6.9.Noforeignproteinsadded.Itisreconstitutedbyadding1mlsteriledistilledwater,spundowntoremoveinsolubleparticles,dividedintosmallaliquots,frozenandstoredatorbelow-20°C.

Applications:

ELISA,Immunocytochemistry,Immunohistochemistry(paraffin),Dotblot,Immunoblotting.

CrossReactivity:

Inter-speciescross-reactivityisanormalfeatureofantibodiestoimmunoglobulins,sinceIgofdifferentspeciesfrequentlyshareantigenicdeterminants.Cross-reactivityofthisimmunoconjugatehasnotbeentestedindetail.

Specificity:

Biotin-conjugatedIgGfractionofpolyclonalGoatantiSerumtoDogIgA,Fcspecific

Storage:

Thelyophilizedconjugateisshippedatambienttemperatureandmaybestoredat+4°C;prolongedstorageatorbelow-20°C.Priortouse,analiquotisthawedslowlyatambienttemperature,spundownagainandusedtoprepareworkingdilutionsbyaddingsterilephosphatebufferedsaline(PBS,pH7.2).Repeatedthawingandfreezingshouldbeavoided.Workingdilutionsshouldbestoredat+4°C,notrefrozen,andpreferablyusedthesameday.Ifaslightprecipitationoccursuponstorage,thisshouldberemovedbycentrifugation.Itwillnotaffecttheperformanceoftheimmunoconjugate.

Caution:

ThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-MubioacceptsnoliABIlityforanyinaccuraciesoromissionsinthisinformation. Nordic的纯化的牛IgG可以在各种免疫测定（包括免疫扩散，免疫电泳，ELISA，Western印迹，斑点免疫结合测定（DIBA），血细胞凝集和细胞结合测定）中用作参考抗原，校准物，包被蛋白和封闭剂。该产品仅用于研究目的，并且用于体外测试，不适用于涉及人或动物的诊断或治疗程序。它可能包含有害成分。请参阅安全数据表（SDS）了解更多信息和正确的处理程序。根据当地法规处理产品残留物。此数据表的准确性尽可能合理，但Nordic-MUbio对本信息中的任何准确性或遗漏概不负责。