Background:

ThereactivityoftheantiserumisdirectedtotheFcandFabsubunitsoftheIgGmolecule.ItincludesacertaindegreeofreactivitywithotherimmunoglobulinsviathecommonFabportion.Itdoesnotreactwithanynon-Igproteiningoatserum,astestedbyimmunoelectrophoresisanddoublerADIalimmunodiffusion.ToidentifyandmeasureIgG,antigenorantibody,atthecellularandsubcellularlevelbyimmunofluorescencestainingofappropriatelytreatedcellandtissuesubstrates,andtodemonstratecirculatingantibodiesinSEROdiagnosticmicroBIOLOGyandautoimmunediseases;toidentifyaspecificantigenorimmunecomplexusingareferenceantibodyofgoatorigininthemiddlelayeroftheindirecttestprocedure.Thisimmunoconjugateisnotpre-diluted.Theoptimumworkingdilutionofeachconjugateshouldbeestablishedbytitrationbeforebeingused.Excesslabelledantibodymustbeavoidedbecauseitmaycausehighunspecificbackgroundstainingandinterferewiththespecificsignal.

Source:

PurifiednormalIgGisolatedfrompooledgoatserum.Freund’scompleteadjuvantisusedinthefirststepoftheimmunizationprocedure.

Product:

Horseradishperoxidase-coupledpurifiedhyperimmunedonkeyIgGlyophilizedfromasolutioninphosphatebufferedsaline(PBS,pH7.2).Nopreservativeadded.Itisreconstitutedbyadding1mlsteriledistilledwater,spundowntoremoveinsolubleparticles,dividedintosmallaliquots,frozenandstoredatorbelow-20°C.

Applications:

ELISA,Immunocytochemistry,Immunohistochemistry(paraffin),Dotblot,Immunoblotting.

CrossReactivity:

Inter-speciescross-reactivityisanormalfeatureofantibodiestoimmunoglobulins,sinceIgofdifferentspeciesfrequentlyshareantigenicdeterminants.Cross-reactivityofthisantiserumhasbeentestedindoubleradialimmunodiffusionwiththefollowingresults:bovine++guineapig±mouse±rat±cat±hamster-monkey+sheep++chicken-horse-pigeon-swine-dog±human+rabbit±turkey-Anegativecross-reactionindoubleradialimmunodiffusiondoesnotexcludesomereactioninmoresensitivetechniques.

Specificity:

Horseradishperoxidase-conjugatedIgGfractionofpolyclonalDonkeyantiSerumtoGoatIgG,heavyandlightchains

Storage:

Thelyophilizedconjugateisshippedatambienttemperatureandmaybestoredat+4°C;prolongedstorageatorbelow-20°C.Priortouse,analiquotisthawedslowlyinthedarkatambienttemperature,spundownagainandusedtoprepareworkingdilutionsbyaddingsterilephosphatebufferedsaline(PBS,pH7.2).Repeatedthawingandfreezingshouldbeavoided.Workingdilutionsshouldbestoredat+4°C,notrefrozen,andpreferablyusedthesameday.Ifaslightprecipitationoccursuponstorage,thisshouldberemovedbycentrifugation.Itwillnotaffecttheperformanceoftheimmunoconjugate.

Caution:

ThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-MubioacceptsnoliABIlityforanyinaccuraciesoromissionsinthisinformation. Nordic的纯化的牛IgG可以在各种免疫测定（包括免疫扩散，免疫电泳，ELISA，Western印迹，斑点免疫结合测定（DIBA），血细胞凝集和细胞结合测定）中用作参考抗原，校准物，包被蛋白和封闭剂。该产品仅用于研究目的，并且用于体外测试，不适用于涉及人或动物的诊断或治疗程序。它可能包含有害成分。请参阅安全数据表（SDS）了解更多信息和正确的处理程序。根据当地法规处理产品残留物。此数据表的准确性尽可能合理，但Nordic-MUbio对本信息中的任何准确性或遗漏概不负责。