Background:

Greenfluorescentprotein(GFP)isa27kDproteinwhichwasoriginallyidentifiedinthephotoorgansofAequoreavictoria(A.victoria)jellyfish.GFPisanaturallyfluorescentproteinwhichemitsgreenlightatamaximumwavelengthof509nmwhenexcitedbyblueorUVlight.InA.victoria,calciumionsbindandactivatetheproteinaequorincausingthereleaseofbluefluorescence,whichisthenabsorbedbyGFPresultinginthereleaseofgreenfluorescence.Inthelaboratory,theGFPproteinhasbeenusedextensivelyasareportermoleculetolabel,andstudy,cellularandsubcellularproteinsinlivingcellsusingawiderangeofBIOLOGicalapplications,includingoncology,cardiovasculardiseases,brainresearch,embryologyandplantsciences,justtonameafew.Forthequantitationoftheexpressionofaspecificprotein,taggedwithGFPinthesemodelsystems,antibodiestoGFPhaveproventobeofvalueinimmunoblottingstudiesandELISAprotocols.

Source:

WildtyperecombinantGFP(fromA.victoria)expressedinE.coliandpurifiedbyhighresolutiongelfiltration.

Product:

Eachvialcontains100µg1mg/mlpurifiedrecombinantGFP(wildtype)in10mMTRISbufferpH8.0,containing0.02%sodiumazide.

Specificity:

ThepurityoftherecombinantGFP(wildtype)proteinis>98%,confirmedbySDS-polyacrylamidegelelectrophoresis,specificabsorbancepeaks,HPLCandA397/280ratios.

Storage:

Maintainat-20°Cinundilutedaliquotsforupto24monthsafterdateofreceipt.Avoidrepeatedfreeze-thawcycles.

References:

ChalfieM,TuY,EuskirchenG,WardWW,PrasherDC.(1994)GreenfluorescentproteinasaMarkerforgeneexpression.Science263:802-805.

Caution:

ThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-MubioacceptsnoliABIlityforanyinaccuraciesoromissionsinthisinformation. Nordic的纯化的牛IgG可以在各种免疫测定（包括免疫扩散，免疫电泳，ELISA，Western印迹，斑点免疫结合测定（DIBA），血细胞凝集和细胞结合测定）中用作参考抗原，校准物，包被蛋白和封闭剂。该产品仅用于研究目的，并且用于体外测试，不适用于涉及人或动物的诊断或治疗程序。它可能包含有害成分。请参阅安全数据表（SDS）了解更多信息和正确的处理程序。根据当地法规处理产品残留物。此数据表的准确性尽可能合理，但Nordic-MUbio对本信息中的任何准确性或遗漏概不负责。