Background:
Confirmedbyimmunoelectrophoresisusingantiseratohumanimmunoglobulins.Notlessthan98%asdeterminedbyimmunoelectrophoresis.Testedinaconcentrationof18mg/mlatthelevelofsensitivityoftheappliedimmunoprecipitatingtechniques,thisproductcontainstracesofalbumin.PurifiedhumanIgEmaybeusedasareferenceantigen,calibrator,coatingproteininavarietyofimmunoassaysincludingimmunodiffusion,immunoelectrophoresis,
ELISA,Westernblotting,dotimmunobindingassays(DIBA).
Product:
Lyophilized,stablewhitepowder.NopreservativeaddedAfteropeningthevial,thelyophilizedcontentisreconstitutedbyadding0.25mlofsteriledistilledwater,mixedgentlybyinversionuntilcompletedissolutionisobtained.
Specificity:
ChromatographicallypurifiedmonoclonalHumanIgEkappa.
Storage:
Thisproductisshippedlyophilizedatambienttemperature.Afterarrivalstoreat+4°C.Prolongedstoragemaybeat-20°C.Allowtostandatambienttemperaturefor5-10minutestoreachequilibrium.Reconstitutedsolutionmaybestoredfrozen.Workingdilutionsmaybepreparedbyaddingtherequiredamountofphosphatebufferedsaline(PBS),shouldnotberefrozenandpreferablyusedwithin24hours.Repeatedthawingandfreezingshouldbeavoided.Ifaslightprecipitationoccursuponstorage,thisshouldberemovedbycentrifugationandwillnotaffecttheperformanceoftheproduct.
Caution:
ThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisdatasheetisasaccurateasreasonablyachievable,but
Nordic-Mubioacceptsnoli
ABIlityforanyinaccuraciesoromissionsinthisinformation. Nordic的纯化的牛IgG可以在各种免疫测定(包括免疫扩散,免疫电泳,ELISA,Western印迹,斑点免疫结合测定(DIBA),血细胞凝集和细胞结合测定)中用作参考抗原,校准物,包被蛋白和封闭剂。该产品仅用于研究目的,并且用于体外测试,不适用于涉及人或动物的诊断或治疗程序。它可能包含有害成分。请参阅安全数据表(SDS)了解更多信息和正确的处理程序。根据当地法规处理产品残留物。此数据表的准确性尽可能合理,但Nordic-MUbio对本信息中的任何准确性或遗漏概不负责。