Background:

ConfirmedbyimmunoelectrophoresisanddoublerADIalimmunodiffusion(Ouchterlony).Notlessthan98%asdeterminedbyagargelelectrophoresis,quantitativedensitometryimmunoelectrophoresisanddoubleradialimmunodiffusion(Ouchterlony).PurifiedhumanIgG3kappamaybeusedasareferenceantigen,calibrator,coatingproteinandblockingagentinavarietyofimmunoassaysincludingimmunodiffusion,immunoelectrophoresis,ELISA,Westernblotting,dot-immunobindingassays(DIBA),haemagglutination,andcell-bindingassays.

Product:

Lyophilized,stablewhitepowderNopreservativeaddedThelyophilizedcontentisreconstitutedbyadding0.5mlofsteriledistilledwater,mixedgentlybyinversionuntilcompletedissolutionisobtained.

Specificity:

ChromatographicallypurifiedHumanmyelomaIgG3kappa,allotypeG3m(b).

Storage:

Thisproductisshippedlyophilizedatambienttemperature.Afterarrivalstoreat+4°C.Prolongedstoragemaybeat-20°C.Allowtostandatambienttemperaturefor5-10minutestoreachequilibrium.Reconstitutedsolutionmaybestoredfrozen.Workingdilutionsmaybepreparedbyaddingtherequiredamountofphosphatebufferedsaline(PBS),shouldnotberefrozenandpreferablyusedwithin24hours.Repeatedthawingandfreezingshouldbeavoided.Ifaslightprecipitationoccursuponstorage,thisshouldberemovedbycentrifugationandwillnotaffecttheperformanceoftheproduct.

Caution:

ThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-MubioacceptsnoliABIlityforanyinaccuraciesoromissionsinthisinformation. Nordic的纯化的牛IgG可以在各种免疫测定（包括免疫扩散，免疫电泳，ELISA，Western印迹，斑点免疫结合测定（DIBA），血细胞凝集和细胞结合测定）中用作参考抗原，校准物，包被蛋白和封闭剂。该产品仅用于研究目的，并且用于体外测试，不适用于涉及人或动物的诊断或治疗程序。它可能包含有害成分。请参阅安全数据表（SDS）了解更多信息和正确的处理程序。根据当地法规处理产品残留物。此数据表的准确性尽可能合理，但Nordic-MUbio对本信息中的任何准确性或遗漏概不负责。