Background:

Inimmunoelectrophoresisagainstpooledserumandenrichedserumproteinsfractionsprecipitationcanbeobservedofnotlessthan20individualproteinscomponents.InprecipitatingtechniquesasimmunoelectrophoresisandrADIalimmunodiffusion(Ouchterlony)toidentifytheserumproteinpattern,orthepresenceorabsenceofanindividualcomponent.Toevaluatethepurityofanisolatedserumproteinincludingimmunoglobulins.Sinceimmunoprecipitationdependsonacorrectantigen/antibodyconcentrationratio(zoneofequivalence)inthegelmedium,theproteinanalysisbyimmunoelectrophoresisofserumoranyotherBIOLOGicalfluidorproteinfractionshouldincludedifferentproportionsofthereactants.ItisnotpossIBLetoobtainanoptimalproteinpatterninasingleanalysis.Theelectroendosmosiseffectofdifferenttypesofagaronproteinswithadifferentnetchargecanbeusedtooptimizetheresolutionpowerofthetestsystem.AgarNordicNr.2containssufficientpositivelychargedionstooptimizetheresolutionoftheproteinsinthebeta-gammaregions,whilethealpharegionswillbecomemoredense.Highlypurifiedagar(AgarNordicnr.1)withlowelectroendosmosisfavourstheresolutionoftheproteinsinthealpharegions,whilethemajorcomponentsinthebeta-gammaregioncanstillbeidentified.

Source:

Pooledwholedogserumandpartlypurifiedserumfractions.Freund’scompleteadjuvantisusedinthefirststepoftheimmunizationprocedure.

Product:

Delipidated,heatinactivated,lyophilized,stablewholeantiserum.Nopreservativeadded.TotalproteinandIgGconcentrationsintheantiserumarecomparabletothoseofpoolednormalgoatserum.Noforeignproteinsadded.Reconstitutethelyophilizedantiserumbyadding1mlsteriledistilledwater.

Applications:

Precipitationassays.Inimmunoelectrophoresisuse2μlserumorequivalentagainst120μlantiserum.Indoubleradialimmunodiffusion(Ouchterlony)usearosettearrangementwith10μlantiserumin3mmdiametercenterwelland2μlserumsamples(neatandseriallydilutedin2mmdiameterperipheralwells.Differentbleedingsoftheimmunizedanimalsarepooledtoobtainabroadspectrumbalancedagainstthevaryingconcentrationsoftheindividualserumproteincomponents.

CrossReactivity:

Inter-speciescross-reactivityisanormalfeatureofantibodiestoanimalproteinssincehomologousproteinsofdifferentspeciesfrequentlyshareantigenicdeterminants.ThisantiSerumhasnotbeenadsorbedforsuchcross-reactivity.Consequentlyitisnotspecies-specific.

Specificity:

PrecipitatingpolyclonalGoatantiSerumtoDogSerumproteins

Storage:

Thelyophilizedantiserumisshippedatambienttemperatureandmaybestoredat+4°C;prolongedstorageatorbelow-20°C.Dilutionsmaybepreparedbyaddingphosphatebufferedsaline(PBS,pH7.2).Repeatedthawingandfreezingshouldbeavoided.Ifaslightprecipitationoccursuponstorage,thisshouldberemovedbycentrifugation.Itwillnotaffecttheperformanceoftheantiserum.Dilutedantiserumshouldbestoredat+4°C,notrefrozen,andpreferablyusedthesameday.

Caution:

ThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-MubioacceptsnoliABIlityforanyinaccuraciesoromissionsinthisinformation. Nordic的纯化的牛IgG可以在各种免疫测定（包括免疫扩散，免疫电泳，ELISA，Western印迹，斑点免疫结合测定（DIBA），血细胞凝集和细胞结合测定）中用作参考抗原，校准物，包被蛋白和封闭剂。该产品仅用于研究目的，并且用于体外测试，不适用于涉及人或动物的诊断或治疗程序。它可能包含有害成分。请参阅安全数据表（SDS）了解更多信息和正确的处理程序。根据当地法规处理产品残留物。此数据表的准确性尽可能合理，但Nordic-MUbio对本信息中的任何准确性或遗漏概不负责。