Nordicmubio/Goat anti Dog fibrinogen, conjugated with Horseradish peroxidase/GAD/Fbg/PO

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货号:GAD/Fbg/PO
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品牌:Nordic
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商品描述

Background:

Thereactivityoftheantiserumisrestrictedtofibrinogen.InimmunoelectrophoresisandrADIalimmunodiffusion(Ouchterlony),usingvariousantiserumconcentrationsagainstnormaldogplasmaasingleprecipitinlineisobtainedwhichshowsareactionofidentitywiththeprecipitinlineobtainedwithpurifiedfibrinogen.Noreactionisobtainedwithanyotherplasmaproteincomponentorserum.However,theantiserummayalsoreactwithfibrinmonomers,circulatingfibrinopeptidesandfibrindegradationproducts.Inenzyme-immunocytochemicalandimmunohistochemicaltechniquesforthedetectionoffibrinogenatthecellularandsubcellularlevelinappropriatelytreatedcellandtissuesubstrates;asdetectionreagentinnon-isotopicmethodologyandsolidphaseimmunochemistry(e.g.ELISA,Westernblotting).Thisimmunoconjugateisnotpre-diluted.Theoptimumworkingdilutionofeachconjugateshouldbeestablishedbytitrationbeforebeingused.Excesslabelledantibodymustbeavoidedbecauseitmaycausehighunspecificbackgroundstainingandinterferewiththespecificsignal.

Source:

Fibrinogen(clottingfactorI)isaheatlABIlebetaglycoproteinpresentinplasma.Itistheprecursoroffibrin,whichisthekeyproteinconstitutingthenetworkofthebloodclot.Thrombinconvertsfibrinogentofibrinbylimitedproteolysis.Fibrinmonomerspolymerizetofibrinwhichisstabilizedbycross-linking.Fibrinogenisisolatedfromfreshplasmaafterremovingprothrombin.Freund’scompleteadjuvantisusedinthefirststepoftheimmunizationprocedure.

Product:

Horseradishperoxidase-coupledpurifiedhyperimmuneIgGlyophilizedfromasolutioninphosphatebufferedsaline(PBS,pH7.2)Nopreservativeadded,asitmayinterferewiththeantibodyactivity.
Itisreconstitutedbyadding1mlsteriledistilledwater,spundowntoremoveinsolubleparticles,dividedintosmallaliquots,frozenandstoredatorbelow-20°C.Priortouse,analiquotisthawedslowlyatambienttemperature,spundownagainandusedtoprepareworkingdilutionsbyaddingsterilephosphatebufferedsaline(PBS,pH7.2).Repeatedthawingandfreezingshouldbeavoided.

Applications:

ELISA,Immunocytochemistry,Immunohistochemistry(paraffin),Dotblot,Immunoblotting.

CrossReactivity:

TheantiSerumdoesnotcross-reactwithanyothercomponentofDogplasma.Inter-speciescrossreactivityisanormalfeatureofantibodiestoplasmaproteinssincetheyfrequentlyshareantigenicdeterminants.Cross-reactivityofthisantiSerumhasnotbeentestedindetail.

Specificity:

Horseradishperoxidase-conjugatedIgGfractionofpolyclonalGoatantiSerumtoDogfibrinogen

Storage:

Thelyophilizedconjugateisshippedatambienttemperatureandmaybestoredat+4°C;prolongedstorageatorbelow-20°C.

Caution:

ThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-Mubioacceptsnoliabilityforanyinaccuraciesoromissionsinthisinformation. Nordic的纯化的牛IgG可以在各种免疫测定(包括免疫扩散,免疫电泳,ELISA,Western印迹,斑点免疫结合测定(DIBA),血细胞凝集和细胞结合测定)中用作参考抗原,校准物,包被蛋白和封闭剂。该产品仅用于研究目的,并且用于体外测试,不适用于涉及人或动物的诊断或治疗程序。它可能包含有害成分。请参阅安全数据表(SDS)了解更多信息和正确的处理程序。根据当地法规处理产品残留物。此数据表的准确性尽可能合理,但Nordic-MUbio对本信息中的任何准确性或遗漏概不负责。