Background:

Indole-3-butyricacid(IBA)isaplanthormonebelongingtothegroupofauxins.Itisusedtostimulaterootformationinplantcuttings.AccordingtonewECregulations,IBAlevelsshouldapproachzeroatthetimetheplantletsareenteringthemarket.InordertobeabletomeasureIBAinsoilsamples,polyclonalantibodiesandacorrespondingELISAwasdeveloped.ThehighlypurifiedBSA-Indole-3-butyricacidconjugatecanbeusedasastandardinantibody-basedtests.

Product:

Eachvialcontains100µg1mg/mlBSA-Indole-3-butyricacidconjugateinPBS,pH7.4.
TheconjugatewaspurifiedandisolatedusingaBioradEcono-Pac10DGcolumnwithHSBasmobilephaseaccordingtotherecommendationsofthemanufacturer.

Applications:

TheBSA-Indole-3-butyricacidconjugateissuitableasastandardforuseinWesternblottingandELISA.Optimalproteindilutionsforthedifferentapplicationsshouldbedeterminedbytitration.

Storage:

Maintainat-20°Cinundilutedaliquotsforupto24monthsafterdateofreceipt.Avoidrepeatedfreeze-thawcycles.

References:

MeulenbergEP(2015)ThedevelopmentandapplicationofELISAfor3-Indole-butyricacid.ImmunochemImmunopathol:OpenAccess1:1000104.

Caution:

ThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-MubioacceptsnoliABIlityforanyinaccuraciesoromissionsinthisinformation. Nordic的纯化的牛IgG可以在各种免疫测定（包括免疫扩散，免疫电泳，ELISA，Western印迹，斑点免疫结合测定（DIBA），血细胞凝集和细胞结合测定）中用作参考抗原，校准物，包被蛋白和封闭剂。该产品仅用于研究目的，并且用于体外测试，不适用于涉及人或动物的诊断或治疗程序。它可能包含有害成分。请参阅安全数据表（SDS）了解更多信息和正确的处理程序。根据当地法规处理产品残留物。此数据表的准确性尽可能合理，但Nordic-MUbio对本信息中的任何准确性或遗漏概不负责。