Background:

ThisreadytouseNegativeControlreagentcontainsacombinationoffluoresceinandphycoerythrinconjugatedmouseimmunoglobulinmoleculesofIgG1isotype,whichhavebeenselectedonthebasisoftheirbindingcharacteristics:nospecificbindingtohumanintracellularorcellsurfaceantigens,samelowrangeofnonspecificbindingtohumanleukocytesasotherCOMBI-IC-Reagents.LikeallotherCOMBI-IC-reagents,thisreagentshouldbeusedincombinationwithourFIX&PERMCellPermeABIlizationKit(Cat.No.GAS-002)TheseisotypecontrolIgG1aresuitableasnegativecontrolstobeusedincombinationwithCOMBI-ICreagentsforthe:-EnumerationofMyeloidCells-AnalysisofMyeloidDifferentiationStage-EnumerationofB-cellsandPrecursors-EnumerationofT-cellsandPrecursors-AnalysisofLeukemiaCells-AnalysisofImmunodeficiencyStatesResultsmustbeputwithinthecontextofotherdiagnostictestsaswellastheclinicalhistoryofthepatientbyacertifiedprofessionalbeforefinalinterpretation.

Product:

1mlofFITC-andPE-conjugatedVI-APinPBSpH7.2,1%BSA,and0.05%NaN3,approximately50tests.

Applications:

PermeabilizationandStainingProcedure-IncombinationwithourPermeabilizationKitFIX&PERM(Cat.No.GAS-002)intracellularisotypecontrolscanbeeasilystainedincellsUSPensions.-Foreachsampletobeanalyzedadd50µlofwholeblood,bonemarrowormononuclearcellsuspensionina5mltube-Add100µlofReagentA(FixationMedium,storedandusedatroomtemperature)-Incubatefor15minutesatroomtemperature-Add5mlphosphatebufferedsalineandcentrifugecellsfor5minutesat300g-Removesupernatantandaddtocellpellet100µlReagentB(PermeabilizationMedium)and20µlofCOMBI-ICNegativeControlantibodyconjugate.-Vortexatlowspeedfor1-2seconds-Incubatefor15minutesatroomtemperature-Washcellswithphosphatebufferedsalineasdescribedabove-Removesupernatantandresuspendcellsinsheathfluidforimmediateanalysisorresuspendcellsin0.5ml1.0%formaldehydeandstorethemat2-8°Cinthedark.Analyzefixedcellswithin24hours.

Specificity:

VI-APreactswithcalfintestinealkalinephosphataseanddoesnotshowcross-reactivitywithhumanproteins.

Storage:

Nordic-Mubiomonoclonalantibodyreagentscontainoptimalconcentrationsofaffinity-purifiedantibody.Forstabilityreasonsthismonoclonalantibodysolutioncontainssodiumazide.Thesereagentsshouldbestoredat2-8°C(DONOTFREEZE!)andprotectedfromprolongedexposuretolight.Stabilityofthereagent:Pleaserefertotheexpirydateprintedontothevial.Theuseofthereagentaftertheexpirationdateisnotrecommended.

References:

1.J.L.Clarke,W.Watkins,JBiolChem271,10317(1996).
2.R.N.Knibbsetal.,JCellBiol133,911(1996).
3.B.Kniepetal.,JBiochem(Tokyo)119,456(1996).
4.A.J.Wagers,L.M.Stoolman,R.Kannagi,R.Craig,G.S.Kansas,JImmunol159,1917(1997).
5.M.Noguchi,N.Sato,H.Sugimori,K.Mori,K.Oshimi,LeukRes25,847(2001).
6.W.M.Watkins,J.L.Clarke,AdvExpMedBiol491,231(2001).

Caution:

Forprofessionalusersonly.Thisreagentcontainssodiumazide.Toavoidthedevelopmentofhazardousconditions,reagentscontainingazideshouldbedilutedinrunningwaterpriortobediscarded.SimilartotheworkwithotherBIOLOGicalproducts,properhandlingproceduresarerecommended. Nordic的纯化的牛IgG可以在各种免疫测定（包括免疫扩散，免疫电泳，ELISA，Western印迹，斑点免疫结合测定（DIBA），血细胞凝集和细胞结合测定）中用作参考抗原，校准物，包被蛋白和封闭剂。该产品仅用于研究目的，并且用于体外测试，不适用于涉及人或动物的诊断或治疗程序。它可能包含有害成分。请参阅安全数据表（SDS）了解更多信息和正确的处理程序。根据当地法规处理产品残留物。此数据表的准确性尽可能合理，但Nordic-MUbio对本信息中的任何准确性或遗漏概不负责。