Background:

Asanefficientgeneralcounterstainforcellandtissuesubstrateswithimmunoconjugateswithalabelcontrastingwiththegreenish-yellowfluorescenceofthisproduct.Thepurposeofcounterstainingistoenhancetheopticalcontrastoftheimmunospecificstainingagainstacounterstainedbackground.

Product:

FluorochromecoupledpurifiedrabbitIgGinphosphatebufferedsaline(pH7.2).Nopreservativeadded

Applications:

ELISA,Immunocytochemistry,Immunohistochemistry(frozen),(In)directimmunofluorescence.Theconjugateisshippedatambienttemperatureandmaybestoredat+4°C,prolongedstorageat-20°C.Reconstitutethelyophilizedantiserumbyadding1mlsteriledistilledwater.Spindowntoremoveinsolubleparticles,divideintosmallaliquots,andstorefrozenatorbelow-20°C.Priortouseanaliquotisthawedatambienttemperature,spundownagain,andusedtoprepareworkingdilutionsbyaddingsterilePBSpH7.2).Repeatedthawingandfreezingshouldbeavoided.Ifaslightprecipitationoccursuponstorage,thisshouldberemovedbycentrifugation.Itwillnotaffecttheperformanceoftheconjugate.Dilutedproductshouldbestoredat+4°C,notrefrozen,andpreferablyusedthesameday.

Specificity:

Fluoresceinisothiocyanate-conjugatedpolyclonalRabbitIgG.

Caution:

ThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-MubioacceptsnoliABIlityforanyinaccuraciesoromissionsinthisinformation. Nordic的纯化的牛IgG可以在各种免疫测定（包括免疫扩散，免疫电泳，ELISA，Western印迹，斑点免疫结合测定（DIBA），血细胞凝集和细胞结合测定）中用作参考抗原，校准物，包被蛋白和封闭剂。该产品仅用于研究目的，并且用于体外测试，不适用于涉及人或动物的诊断或治疗程序。它可能包含有害成分。请参阅安全数据表（SDS）了解更多信息和正确的处理程序。根据当地法规处理产品残留物。此数据表的准确性尽可能合理，但Nordic-MUbio对本信息中的任何准确性或遗漏概不负责。