Background:

Thereactivityoftheantiserumisrestrictedtofibrinogen.InimmunoelectrophoresisandrADIalimmunodiffusion(Ouchterlony),usingvariousantiserumconcentrationsagainstnormaldogplasmaasingleprecipitinlineisobtainedwhichshowsareactionofidentitywiththeprecipitinlineobtainedwithpurifiedfibrinogen.Noreactionisobtainedwithanyotherplasmaproteincomponentorserum.However,theantiserummayalsoreactwithfibrinmonomers,circulatingfibrinopeptidesandfibrindegradationproducts.Inenzyme-immunocytochemicalandimmunohistochemicaltechniquesforthedetectionoffibrinogenatthecellularandsubcellularlevelinappropriatelytreatedcellandtissuesubstrates;asdetectionreagentinnon-isotopicmethodologyandsolidphaseimmunochemistry(e.g.ELISA,Westernblotting).Thisimmunoconjugateisnotpre-diluted.Theoptimumworkingdilutionofeachconjugateshouldbeestablishedbytitrationbeforebeingused.Excesslabelledantibodymustbeavoidedbecauseitmaycausehighunspecificbackgroundstainingandinterferewiththespecificsignal.

Source:

Fibrinogen(clottingfactorI)isaheatlABIlebetaglycoproteinpresentinplasma.Itistheprecursoroffibrin,whichisthekeyproteinconstitutingthenetworkofthebloodclot.Thrombinconvertsfibrinogentofibrinbylimitedproteolysis.Fibrinmonomerspolymerizetofibrinwhichisstabilizedbycross-linking.Fibrinogenisisolatedfromfreshplasmaafterremovingprothrombin.Freund’scompleteadjuvantisusedinthefirststepoftheimmunizationprocedure.

Product:

Horseradishperoxidase-coupledpurifiedhyperimmuneIgGlyophilizedfromasolutioninphosphatebufferedsaline(PBS,pH7.2)Nopreservativeadded,asitmayinterferewiththeantibodyactivity.
Itisreconstitutedbyadding1mlsteriledistilledwater,spundowntoremoveinsolubleparticles,dividedintosmallaliquots,frozenandstoredatorbelow-20°C.Priortouse,analiquotisthawedslowlyatambienttemperature,spundownagainandusedtoprepareworkingdilutionsbyaddingsterilephosphatebufferedsaline(PBS,pH7.2).Repeatedthawingandfreezingshouldbeavoided.

Applications:

ELISA,Immunocytochemistry,Immunohistochemistry(paraffin),Dotblot,Immunoblotting.

CrossReactivity:

TheantiSerumdoesnotcross-reactwithanyothercomponentofDogplasma.Inter-speciescrossreactivityisanormalfeatureofantibodiestoplasmaproteinssincetheyfrequentlyshareantigenicdeterminants.Cross-reactivityofthisantiSerumhasnotbeentestedindetail.

Specificity:

Horseradishperoxidase-conjugatedIgGfractionofpolyclonalGoatantiSerumtoDogfibrinogen

Storage:

Thelyophilizedconjugateisshippedatambienttemperatureandmaybestoredat+4°C;prolongedstorageatorbelow-20°C.

Caution:

ThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-Mubioacceptsnoliabilityforanyinaccuraciesoromissionsinthisinformation. Nordic的纯化的牛IgG可以在各种免疫测定（包括免疫扩散，免疫电泳，ELISA，Western印迹，斑点免疫结合测定（DIBA），血细胞凝集和细胞结合测定）中用作参考抗原，校准物，包被蛋白和封闭剂。该产品仅用于研究目的，并且用于体外测试，不适用于涉及人或动物的诊断或治疗程序。它可能包含有害成分。请参阅安全数据表（SDS）了解更多信息和正确的处理程序。根据当地法规处理产品残留物。此数据表的准确性尽可能合理，但Nordic-MUbio对本信息中的任何准确性或遗漏概不负责。