Species:Mouse MonoclonalClone:DO-1Isotype:IgG2aMW:53 kDSpecies Reactivity:Human, Monkey, Cow, and weak reaction with Mouse and Rat.Positive Control: About 50% of breast carcinomas are positive for p53, especially those lacking estrogen and/or progesterone receptor, or with high proliferation index.Specificity:Recognizes a 53kDa phosphoprotein, identified as p53 suppressor gene product. It reacts with mutant as well as wild form of p53. Its epitope maps within the N-terminus (aa37-45) of p53. Monoclonal antibody Pab 1801 does not block the binding of DO-1 to p53 in an ELISAtest. PolyTek，抗兔聚合HRP已开发，具有成本效益，可提供最清洁，最一致的染色。该系统基于聚合的过氧化物酶标记，该标记从等式中消除了生物素，从而消除了背景染色的主要原因。