Species:MouseImmunogen:Purified involucrin from human keratinocytesClone:SY5Isotype:IgG1, kappaSpecies Reactivity:Human, Gorilla, Owl monkey, Pig, and Dog. Does not react with Mouse.Others not known.Positive Control:MCF-7 cells.Localized to upper spinous and granular layers in normal skin.Specificity:This antibody recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this antibody reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Status:RUO PolyTek，抗兔聚合HRP已开发，具有成本效益，可提供最清洁，最一致的染色。该系统基于聚合的过氧化物酶标记，该标记从等式中消除了生物素，从而消除了背景染色的主要原因。