Species:Rabbit PolyclonalClone:PolyclonalMW:270 kDSpecies Reactivity:HumanPositive Control:Blood vessels in skin or intestineSpecificity:Recognizes a multimeric glycoprotein of 270kDa, identified as factor VIII related antigen or von Willebrand factor. This protein has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. Von Willebrand factor is synthesized by endothelial cells and stored in the Weibel-Palade granules. It mediates platelet adhesion to injured vessel walls and serves as a carrier and stabilizer for coagulation factor VIII. ScyTek's antibody reacts specifically with the endothelial cells of normal, reactive, and neoplastic blood and lymphatic vessels and shows a finely granular cytoplasmic staining. It also reacts with endocardium, platelets and megakaryocytes. Von Willebrand factor is one of the most useful markers to identify endothelial (or megakaryocytic) lineage of neoplasms. However, because not all endothelial cells synthesize (or store) this molecule, about 30% of tumors of vascular origin fail to stain for factor VIII related antigen, regardless of whether they are benign or malignant. PolyTek，抗兔聚合HRP已开发，具有成本效益，可提供最清洁，最一致的染色。该系统基于聚合的过氧化物酶标记，该标记从等式中消除了生物素，从而消除了背景染色的主要原因。