ProductDescriptionSize:100μlAbType:MonoclonalReactivity:AllImmunogen:NitratedKLHHost:MOUSE

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ProductDescriptionThemouse-derivedanti-3NTantibodyispurifiedwithProteinG.Itspecificallyrecognizesproteinswithtyrosinenitratedresiduesbutnotthetyrosinephosphorylatedornon-modifiedtyrosineresidues.BesidescommonapplicationsinWesternblottingandimmunoprecipitation,theantibodyhasbeenwellutilizedforproteomicscreeningtotyrosinenitratedsubstratesfromprotease-digestedproteinsbothinprokaryoticandeukaryoticcells.Source/PurificationThisproductisproducedbyimmunizingmicewithnitratedKLH.TheasciteswaspurifiedbyproteinG.SpecificityAllnitratedproteinsexpectedRecommendedApplicationsELISA,WB,IP*WB=Westernblotting; IP=ImmunoprecipitationRecommendedantibodydilutionWB:1:1000ImportantForWesternblotting,incubatemembranewithdilutedantibodyin5%BSA,1xTBS,0.1%Tween-20at4℃withgentleshaking,overnight.Useatanassaydependentconcentration.Optimaldilutions/concentrationsshouldbedeterminedbytheenduser.Notyettestedinotherapplications.Dotblotting analysisonindicatedamountofpeptidesusing3-nitrotyrosinemousemAb.Thelistofpeptidesisincludedinthetableontheright.Westernblot analysison30μgofextractsfromHeLaandMEFwholecelllysates,untreatedortreatedwithperoxynitrite(3mM,30mins)orpervanadate(3mM,30mins),usinganti-3-nitrotyrosinemouseAntibody(FigureB)oranti-phospho-TyrosinerabbitpAb(PTM-702)(FigureC).ScientificDescriptionNitrotyrosineisaproductoftyrosinenitrationmediatedbyreactivenitrogenspeciessuchasperoxynitriteanionandnitrogendioxide.ItisverystableandsoissuitableforanalysisinBIOLOGicalsamplesuchasplasma,lungaspirants-BALF(Bronchoalveolarliningfluid)andurine.IncreasednitrotyrosinelevelshavebeenassociatedwithseveralphysiologicalandpathologicalconditionsandisconsideredaMarkerofNO-dependent,reactivenitrogenspecies-inducednitrativestress.ResearchshowsthatnitrotyrosinelevelscanbereducedbyN-acetylcysteinewhichisaprecursortoglutathione,oneofthebody’sprimaryendogenousantioxidants.Storage&StABIlityTheantibodyisdeliveredinlyophilizedpowder.Uponreceipt,pleasecentrifugetheantibodyat12,000xgfor20seconds,andthenreconstituteitwiththeantibodystabilizerprovided.ThestabilizercontainsPBS,50%glycerol,and0.01%sodiumazide.Pleasestoretheantibodyat-20°C.Avoidrepeatedfreeze/thawcycles.Antibodyisstablefor12monthsfromdateofreconstitution.Equilibratetheantibodytoroomtemperaturefor2minutesandmixbygentlypipettingbeforeuse.**Researchpurposesonly.Theproductisnotintendedtobeusedfortherapeuticordiagnosticpurposesinhumansoranimals. ICL蛋白质参考标准品和同工型12表位标签控制货号：45-LYS-Z 主持人：重组 反应性：6-HIS标签，C-Myc标签，E标签，Flag标签TM，Glu Glu标签，GST标签，HA标签，HSV标签，S标签，T7标签，V5标签，VSV-g标签 目标：12个标签蛋白质标记0.2毫升$ 175.00犬心丝虫抗原货号：RA-40HRT 主机：N / A 目标：犬心丝虫1.0毫克$ 350.00猫肝珠蛋白-纯化货号：AG-20HPT 主持人：猫 反应性：猫 目标：单珠蛋白1毫克$ 725.00富含猫IgG货号：RA-20G 主持人：猫 反应性：猫 目标：IgG h + 120.0毫克$ 145.00纯化的猫IgG货号：AG-20G 主持人：猫 反应性：猫 目标：IgG h + 15.0毫克$ 145.00Cat SAA纯化货号：AG-20SAA-Z 主持人：重组 反应性：猫 目标：血清淀粉样蛋白A0.1毫克$ 225.00鸡肉富含IgY货号：RA-30Y 主持人：鸡肉 反应性：鸡肉 目标：IgY h + 125毫克$ 115.00鸡肉IgY纯化目录编号：AG-30Y 主持人：鸡肉 反应性：鸡肉 目标：IgY h + 15.0毫克$ 95.00冠状病毒（SARS-CoV-2）核蛋白-纯化货号：AG55-0250-Z 主持人：重组 反应性：COVID-19（NP） 目标：SARS-CoV-2（NP）0.1毫克$ 375.00冠状病毒（SARS-CoV-2）穗状NTD-从HEK293纯化货号：AG55-0253-Z 主持人：人类 反应性：COVID-19（Spike NTD） 目标：SARS-CoV-2（NTD）0.1毫克$ 375.00