SUMO-conjugating enzyme UBC9, also called UBE2I, is a protein that in humans is encoded by the UBE2I gene. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. UBE2I could fully complement the mutant phenotype of a yeast ubc9 mutant strain. This gene may play a similar role via interaction with WT1, which is able to impose a block to cell cycle progression in eukaryotic cells. Additionally, it could support the growth of yeast ubc9 temperature-sensitive mutants at nonpermissive temperatures, indicating that the gene is a functional homolog of yeast ubc9. The protein is specifically associated with FHIT, such as FHIT may be involved in cell cycle control through its interaction with UBE2I.
The stated application concentrations are suggested starting amounts.Titration of the UBC9 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
An amino acid sequence from the C-terminus of human UBC9/UBE2I (IKQILLGIQELLNE) was used as the immunogen for this UBC9 antibody (100% homologous in human, mouse and rat).
After reconstitution, the UBC9 antibody can be stored for up to one month at4oC.For long-term, aliquot and store at -20oC.Avoid repeated freezing and thawing.