Receptor Activator of Nuclear Factor K B, also known as TRANCE Receptor and Tumor necrosis factor receptor superfamily member 11A (TNFRSF11A), is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is also expressed on dendritic cells and facilitates immune signaling. RANKL (Receptor Activator for Nuclear Factor K B Ligand) is found on the surface of stromal cells, osteoblasts, and T cells. By analysis of somatic cell and radiation hybrid panels, Anderson et al.(1997) mapped the RANK gene to 18q22.1.
The stated application concentrations are suggested starting amounts.Titration of the RANK antibody may be required due to differences in protocols and secondary/substrate sensitivity.
An amino acid sequence from the N-terminus of human RANK (QIAPPCTSEKHYEHLG) was used as the immunogen for this RANK antibody.
After reconstitution, the RANK antibody can be stored for up to one month at4oC.For long-term, aliquot and store at -20oC.Avoid repeated freezing and thawing.
