This gene was identified as a tumor suppressor that ismutated in a large number of cancers at high frequency. The proteinencoded this gene is a phosphatidylinositol-3,4,5-trisphosphate3-phosphatase. It contains a tensin like domain as well as acatalytic domain similar to that of the dual specificity proteintyrosine phosphatases. Unlike most of the protein tyrosinephosphatases, this protein preferentially dephosphorylatesphosphoinositide substrates. It negatively regulates intracellularlevels of phosphatidylinositol-3,4,5-trisphosphate in cells andfunctions as a tumor suppressor by negatively regulating AKT/PKBsignaling pathway.
Titration of the phospho-PTEN antibody may be required due to differences in protocols and secondary/substrate sensitivity.
This phospho-PTEN antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT382 of human PTEN.
Aliquot the phospho-PTEN antibody and store frozen at -20oC or colder.Avoid repeated freeze-thaw cycles.